vetpatolВетеринарная патологияRussian Journal of Veterinary Pathology2949-4826Don State Technical University10.23947/2949-4826-2026-25-2-54-60GIVVOKvetpatol-2149Research ArticleПатология животных, морфология, физиология, фармакология и токсикологияAnimal pathology, morphology, physiology, pharmacology and toxicologyЭкспрессия vimentin, α-SMA и CD34 в злокачественных типах опухолей молочной железы у собакExpression of Vimentin, α-SMA, and CD34 in Malignant Mammary Tumors in DogsДарбишеваА. А.DarbishevaA. A.

Дарбишева Алина Андреевна, аспирант кафедры паразитологии и ветсанэкспертизы, анатомии и патанатомии им. С.Н. Никольского института ветеринарии и биотехнологий

355017, г. Ставрополь, пер. Зоотехнический, д. 12

Alina A. Darbisheva, Post Graduate Degree Student of the Parasitology and Veterinary-Sanitary Inspection, Anatomy and Pathoanatomy Department Named after S.N. Nikolsky

12, Zootechnichesky Lane, Stavropol, 355017

darbisevaalina@gmail.comhttps://orcid.org/0000-0003-0247-8633ДилековаО. В.DilekovaO. V.

Дилекова Ольга Владимировна, доктор биологических наук, доцент кафедры паразитологии и ветсанэкспертизы, анатомии и патанатомии им. С.Н. Никольского института ветеринарии и биотехнологий

355017, г. Ставрополь, пер. Зоотехнический, д. 12

Olga V. Dilekova, Dr.Sci. (Biology), Associate Professor of the Parasitology and Veterinary-Sanitary Inspection, Anatomy and Pathoanatomy Department Named after S.N. Nikolsky

12, Zootechnichesky Lane, Stavropol, 355017

dilekova2009@yandex.ruСтавропольский государственный аграрный университетРоссияStavropol State Agrarian UniversityRussian Federation2026230720262525460Copyright © Дарбишева А.А., Дилекова О.В., 20262026Дарбишева А.А., Дилекова О.В.Darbisheva A.A., Dilekova O.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vetpat.ru/jour/article/view/2149

Введение. На данном этапе изучения опухолей молочной железы микроокружение рассматривается как ключевой элемент, способствующий формированию и метастазированию неоплазий. В связи с этим наибольшее внимание уделяется клеточному составу микроокружения опухоли молочной железы, в котором наиболее распространены стромальные фибробласты CAFs. Однако в ветеринарной онкологии маркеры стромы злокачественных опухолей молочной железы изучены недостаточно. Цель исследования — изучить экспрессию онкомаркерных белков vimentin, α-SMA, CD34 в стромальном компоненте микроокружения злокачественных опухолей молочной железы у собак.Материалы и методы. Объектом исследования, проведенного в 2022–2024 гг., являлись самки собак разных пород и возраста (n=26). Материал исследования — опухоль молочной железы. Комплекс гистологических и иммуногистохимических исследований проведен по общепринятым методикам.Результаты исследования. Установлено, что при разных гистологических типах и степени злокачественности опухолей молочной железы у собак клетки фибробластического дифферона обладают высокой экспрессией виментина и α-SMA и низкой экспрессией CD34, что указывает на их дифференцировку в CAFs.Обсуждение и заключение. Полученные результаты можно использовать в качестве качестве базовых прикладных данных при изучении канцерогенеза в ветеринарной онкологии.

Introduction

Introduction. At the contemporary stage of mammary tumor studies, the microenvironment is considered a key factor contributing to neoplasia formation and metastasis. Therefore, the greatest attention is paid to the cellular composition of the mammary tumor microenvironment, in which the most widespread elements are the stromal cancer-associated fibroblasts (CAFs). However, in veterinary oncology, stromal markers of malignant mammary tumors are insufficiently studied. The aim of the present study is to investigate the expression of tumor marker proteins, i.e. vimentin, α-SMA, and CD34, in the stroma of the malignant mammary tumor microenvironments in dogs.

Materials and Methods

Materials and Methods. The objects of the study that was conducted in the period from 2022 to 2024 were the female dogs of various breeds and ages (n=26). The materials studied were the mammary tumors. A comprehensive range of histological and immunohistochemical studies was conducted in compliance with the standard techniques.Results. It has been established that in different histological types and degrees of canine mammary tumor malignancy, the fibroblastic differentiation lineage cells demonstrate a high expression of vimentin and α-SMA and a low expression of CD34, which indicates their differentiation into CAFs.Discussion and Conclusion. The obtained results can be used as basic applied data in the study of carcinogenesis in veterinary oncology.

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The authors declare that there are no conflicts of interest present.