The aim of the study was to study the effect of a broad-spectrum antibiotic - enrofloxacin on the qualitative and quantitative composition of the intestinal microflora of honey bees. The experiments were carried out in two groups - experimental and control. In each group, 50 insects were examined. During the whole experiment, the bees in the control group were given regular sugar syrup. Bees in the control group were also given sugar syrup with a 10 % of antibiotic. Carbohydrate forage in both groups was given 3 times with an interval of 7 days. Before the start of the experiment and 24 hours ather the third feeding, the necessary microbiological and bacteriological studies were carried out to determine the amount and type of microflora in the bees intestines. It was found that carbohydrate feeding to the above scheme, namely sugar syrup with the addition of the enrofloxacin, promotes the development of signs of selective dysbiosis in the digestive tract of bees, which is manifested by the almost complete disappearance of the number of gram-positive and gram-negative bacteria and an increase of 0.4-0.7 lg CFU/g quantitative presence of yeasts and fungus