In two farms of the Neklinovsky district in the SEC collective farm «50 years of October» and the SEC kolkhoz «Limanny» conducted bacteriological studies of biological materials from cows and calves, which found the presence of E. coli in the vagina, amniotic fluid after calving and in milk samples. In the SEC collective farm «50 years of October» in the first barn from 14 studied cows isolated culture of Escherichia from amniotic fluid in the amount of 7 cultures, vagina - 7, feces - 6, milk - 8. Examined feces from 14 newborn calves. Studies have shown that from 14 samples of feces of calves was isolated by 11 cultures of E. coli. A total of 70 samples were studied in the first barn, 39 E. coli cultures were isolated. In the third barn from 7 studied cows the causative agent of escherichiosis was isolated from amniotic fluid in the amount of 3 cultures, vagina - 2, feces - 2, milk - 4. Examined feces from neonatal calves 7. Studies have shown that 6 Escherichia cultures were isolated from 7 samples of faeces of calves. A total of 42 samples were studied in the third barn, 17 E. coli cultures were identifled. In SPK collective farm «Estuary» in the barn of the 12 cows studied isolated cultures of E. coli from amniotic fluid of 7 crops of the vagina - 7, feces - 2, - 9 milk. Examined feces from 12 newborn calves. Studies have shown that 11 E. coli cultures were isolated from 12 samples of faeces of calves. A total of 60 samples were examined, 36 E. coli cultures were isolated. Infection with E. coli of newborn calves occurs both intrauterine and aer birth, when newborn calves are watered with the milk of a sick mother, as well as in contact with objects of the environment that are infected with Escherichia. This suggests that the disease in the farm is chronic. The source of the illness echrishiosis are mother cows