ИММУННЫЙ ОТВЕТ ЦЫПЛЯТ НА ВИРУС ГЕРПЕСА ИНДЕЕК
Abstract
The dynamics of the immunocompetent cell response and the intensity of creation of antibodies to Mareks
disease vaccine on the basis of turkey herpesvirus were studied. The lymphocyte migration inhibition test
for determination of antigen-induced migration level (ML) and indirect solid-phase immunosorbent assay
for determination of serum antibody titre (T) were used. It was shown that by day 28 the migration level in
SPF chicks was ML±G=0,38±0,09 and in commercial broiler chicks it was ML±G=0,13±0,059. Moreover, the
antibody mean titre in SPF chicks was 1:190 by day 7 and 1:1445 by day 63. In broiler chicks with the mean
titre of transovarian antibodies 1:8590 by day 7 it was demonstrated that the active immune response was induced
by day 35 (titre 1:358); by day 56 the titre was equal to 1:2692. Coefficients of correlation (R±m) between
lymphocyte migration inhibition test and indirect solid-phase immunosorbent assay were determined
and at the phase of the development of active immune response in SPF chicks and broiler chicks they made
up 0,814±0,111 and 0,840±0,136, correspondingly.
disease vaccine on the basis of turkey herpesvirus were studied. The lymphocyte migration inhibition test
for determination of antigen-induced migration level (ML) and indirect solid-phase immunosorbent assay
for determination of serum antibody titre (T) were used. It was shown that by day 28 the migration level in
SPF chicks was ML±G=0,38±0,09 and in commercial broiler chicks it was ML±G=0,13±0,059. Moreover, the
antibody mean titre in SPF chicks was 1:190 by day 7 and 1:1445 by day 63. In broiler chicks with the mean
titre of transovarian antibodies 1:8590 by day 7 it was demonstrated that the active immune response was induced
by day 35 (titre 1:358); by day 56 the titre was equal to 1:2692. Coefficients of correlation (R±m) between
lymphocyte migration inhibition test and indirect solid-phase immunosorbent assay were determined
and at the phase of the development of active immune response in SPF chicks and broiler chicks they made
up 0,814±0,111 and 0,840±0,136, correspondingly.
About the Authors
А. Пяткина
ФГУ «Федеральный центр охраны здоровья животных»
Russian Federation
Russian Federation
Ш. Куляшбекова
ФГУ «Федеральный центр охраны здоровья животных»
Russian Federation
Russian Federation
А. Меньщикова
ФГУ «Федеральный центр охраны здоровья животных»
Russian Federation
Russian Federation
А. Борисов
ФГУ «Федеральный центр охраны здоровья животных»
Russian Federation
Russian Federation
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