EXPRESSION OF ONCOMARKER PROTEINS IN MAMMARY GLAND TUMOR CELLS IN CATS
https://doi.org/10.25690/VETPAT.2022.72.67.007
Abstract
At the present stage of the study of carcinogenesis, the main mechanism of genetically determined changes leading to uncontrolled cell malignancy is based on molecular research. The greatest interest is given to the study of proteins included in the region of nucleolar organizers (Ag-NROR-proteins) and supporting the biogenesis and transcription of ribosomes. The main biomarkers are: nucleolin/NCL (the phosphoprotein involved in the processing step), nucleophosmin/B23 (the family of non-histone proteins that maintain genome stability), fibrillarin/FBL (the main regulator of several stages of ribosome biogenesis). In the presented work, nucleolar chaperones were studied: nucleolin/NCL, nucleophosmin/B 23, fibrillarin/FBL in mammary gland tumor cells in cats (n = 16). The review considers the results of an immunohistochemical study, which revealed the production of these proteins and their localization in cells. During the study, it was revealed that the location of NCL, B23 and FBL is nucleolar and nuclear, from moderate (+2) to intense (+3). In addition, the localization of NCL was visualized in the cytoplasm of cells, which characterizes this biomarker as a chaperone for protein transfer. The studied transcription proteins lead to chromosomal aberrations inside cells, and, as a consequence, to malignant transformation
About the Authors
V. V. Mitenko
«Stavropol State Agrarian University»
Russian Federation
Russian Federation
O. V. Dilekova
«Stavropol State Agrarian University»
Russian Federation
Russian Federation
References
1. Мустафин Р. Н. Транспозонная гипотеза канцерогенеза /DOI: 10.23868/202110002 Гены & Клетки, том XVI - 2021. - № 3. - С. 8-15.2.
2. Высоцкая И. В. Актуальные вопросы канцерогенеза / И. В. Высоцкая, В. П. Летягин, М. А. Шабанов, В. Ю. Кирсанов и др. // Клиническая онкогематология. - 2019. - С. 101-104.
3. Айхлин Н. Т. Аргирофильные белки областей ядрышковых организаторов - маркеры скорости клеточной пролиферации: Обзор / Н. Т. Айхлин, И. А. Букаева, Н. А. Пробатова // Арх. патол. - 2006. 68 (3): 47-51
4. Бобров И. П. Ядрышковый аппарат и скорость клеточной пролиферации при предраковых заболеваниях и раке молочной железы / И. П. Бобров, А. Ю. Долгатов, А. Ф. Лазарев и др. // Сибирский онкол. журн. - 2008. Приложение № 2: 27-9
5. Wang H. BRCA2 and nucleophosmin co-regulate centrosome amplification and form a complex with Rho effector kinase ROCK2 / H. Wang, K. Takenaka, A. Nakanishi [et al.] // Cancer Res. - 2010; 71 (1): 68-77
6. Мухарьямова К. Ш. Особенности формирования и функционирования ядрышкового аппарата в условиях пространственного разобщения хромосом: дис. … канд. биол. наук (03.00.11 - эмбриология, гистология и цитология / К. Ш. Мухарьямова; рук. работы О. В. Зацепина. - М.: МГУ, 1999. - 56-78 с
7. Ressel1 L. Reduced PTEN protein expression and its prognostic implications in canine and feline mammary tumors / L. Ressel1, F. Millanta, E. Caleri, V. M. Innocenti // Vet. Pathol. - 2009. Sep;46(5):860-8. doi: 10.1354/vp.08-VP-0273-P-FL. Epub 2009 May
8. Sirri V. Amount Variability of Total and Individual Ag-NOR Proteins in Cells Stimulated to Proliferate / V. Sirri, P. Roussel, D. Tref // Institut Jacques Monod, Pani, France (VS, PR, DH-v) and Dipartimento di Patologia Sperimentale, Universita di Bologna, Bologna, Italy (VS, DI; MD). Received for publication December 21, 1994 and in revised form March 29, 1995; accepted April 25, 1995 (4A3563)
9. Зенит-Журавлева Е. Г. Нуклеофозмин и нуклеолин: кодирующие гены и экспрессия в различных тканях животных и человека / Е. Г. Зенит-Журавлева, Е. М. Полковниченко, А. А. Лушникова, Е. М. Трещалина и др. // Молекулярная медицина. - 2012. - № 4
10. Каrhemo P. An extensive tumor array analysis supports tumor suppressive role for nucleophosmin in breast cancer / P. Каrhemo, A. Rivinoja, J. Lundin [et al.] // Am. J. Pathol. - 2011; 179 (2): 1004-14
11. Derenzini M. Structural and Functional Organization of Ribosomal Genes within the Mammalian Cell Nucleolus / M. Derenzini, G. Pasquinelli, Marie-Francoise O’Donohue, D. Ploton [et al.] // - 2006. - С. 131-146
12. Jamison J. M. Nucleolar Changes and Fibrillarin Redistribution Following Apatone Treatment of Human Bladder Carcinoma Cells /j. M. Jamison, J. Gilloteaux, L. Perlaky, M. Thiry, K. Smetana // Journal of Histochemistry & Cytochemistry Debora. - 2010. - C. 635-651
13. Muscatello L. V. HER2 Amplification Status in Feline Mammary Carcinoma: A Tissue Microarray-Fluorescence In Situ Hydridization-Based Study /L. V. Muscatello, Enrico Di Oto, G. Sarli, V. Monti [et al.] // Vet. Pathol. Mar;56(2):230-238. doi: 10.1177/0300985818808531 - 2019г с.34
14. Sayasith K. Molecular characterization of feline COX-2 and expression in feline mammary carcinomas / K. Sayasith, J. Sirois, M. Doré // Vet. Pathol. doi: 10.1354/vp.08-VP-0161-D-FL PMID: 19176489 2009 May;46(3):423-9
15. Soares M. Ki-67 as a Prognostic Factor in Feline Mammary Carcinoma: What Is the Optimal Cutoff Value / M. Soares, R. Ribeiro, S. Carvalho, M. Peleteiro [et al.] // Vet. Pathol. doi: 10.1177/0300985815588606 2016 Jan;53(1):37-43
Review
For citations:
Mitenko V.V., Dilekova O.V. EXPRESSION OF ONCOMARKER PROTEINS IN MAMMARY GLAND TUMOR CELLS IN CATS. Russian Journal of Veterinary Pathology. 2022;(3):45-51. (In Russ.) https://doi.org/10.25690/VETPAT.2022.72.67.007
Views:
69
Email this article 