СПОСОБ ОЦЕНКИ СТРЕССУСТОЙЧИВОСТИ СВИНЕЙ

Abstract

The goal of this study is to suggest a method of evaluation of stress resistance in swine based upon measurements of antioxidant defense and lipid peroxidation. The paper reviews materials on comparison of stress response study results in swine determined using conventional methods and advanced procedure suggested by authors involving PSS determination on the basis of antioxidant defense and lipid peroxidation measures. With the purpose of stress resistance evaluation blood samples were taken from caudal or articular artery in swine at 2 and 6 months old and tested to the characteristics related to antioxidant system and lipid peroxidation state: superoxide dismutase activity, catalase activity, total blood serum oxidative activity, total antioxidant activity, malondialdehyde content, as well PCR test was performed to detect mutation in ryanodine receptor gene (RYR-1) and determine genotype NN-, Nn - stress resistant, nn - stress-susceptible. Accuracy of individual PSS “-“ or PSS “+” characteristics determination in animals was proved high enough for selection or breeding purposes.