ВЫЯВЛЕНИЕ CHLAMYDOPHILA PECORUM С ПОМОЩЬЮ ПОЛИМЕРАЗНОЙ ЦЕПНОЙ РЕАКЦИИ: ГЕНЕТИЧЕСКОЕ РАЗНООБРАЗИЕ И РАСПРОСТРАНЕНИЕ
Аннотация
was developed. It was demonstrated that Chl. pecorum population circulating in the territory of the Russian
Federation is characterized by high genetic heterogeneity. The examination of 390 samples (nasal swabs
of 0.5 - 7 - month - old calves) from 13 premises of 7 oblasts of the Central Federal Okrug showed that the
herd Chl. pecorum prevalence in calves was 92,3% and the individual prevalence was 22,7%. Statistically
significant association between the resalts of the developed diagnostic test and clinical signs of the respiratory
tract damage in calves was detected.
Об авторах
М. А. ШибаевРоссия
С. А. Дудников
Россия
Л. Б. Прохватилова
Россия
Список литературы
1. Дудников, С.А. Количественная эпизоотология: основы прикладной эпидемиологии и биостатистики / С.А. Дудников. - Владимир: Демиург, 2004. - 460 с.
2. Инфекционная патология животных: в 2т. Т.2. / под ред. А.Я. Самуйленко, Б.В. Соловьева, Е.А. Непоклонова, Е.С. Воронина. - М.: ИКЦ Академкнига, 2006. - 807 с.
3. Обухов, И.Л. Свойства и цикл развития хламидий. Взаимодействие с клеткой - хозяином. (обзор) / И.Л. Обухов // Сельскохозяйственная биология. - 1999. - N 4. - C. 12 - 27.
4. A clinically silent respiratory infection with Chlamydophila spp. in calves is associated with airway obstruction and pulmonary inflammation / J. Jaegera, E. Liebler-Tenorioa, N. Kirschvinkc, [et al.] // Vet. Res. - 2007. - Vol. 38.- P. 711-728.
5. An investigation into the role of Chlamydophila spp. in bovine upper respiratory tract disease / D.F. Twomey, P.C. Griffiths, M.W. Horigan [et al.] // Vet. J. - 2006. - Vol. 171. - P. 574 - 576.
6. Antigenic and morphological differentiation of placental and intestinal isolates of Chlamydia psittaci of ovine origin / P.C. Griffiths, H.L. Philipsb, M. Dawsona, M.J. Clarkson // Vet. Microbiol. - 1992. - Vol. 30, № 2-3. - P. 165-177.
7. Bush, R.M. Molecular evolution of the Chlamydiaceae / R.M. Bush, K.D.E. Everett // Inter. J. Syst. Bacterial. - 2001. - Vol. 51. - P. 203 - 220.
8. Clarkson, M.J. Isolation of faecal chlamydiae from sheep in Britain and their characterisation by cultural properties / M.J Clarkson, H.L. Philips // Vet. J. - 1997. - Vol. 153(3). - P. 307-311.
9. Comparison of five tests for the detection of antibodies against chlamydial (enzootic) abortion of ewes / G.E. Jones, J.C. Low, J. Machell [et al.] // Vet. Rec. - 1997. - Vol. 141. - P. 164-168.
10. Everett, K.D.E. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms / K.D.E. Everett, R.M. Bush, A.A. Andersen // Inter. J. Syst. Bacterial. - 1999. - Vol. 49. - P. 415 - 440.
11. Everett, K.D.E. Identification of nine species of the Chlamydiaceae using PCR-RELP / K.D.E. Everett, A.A. Andersen // Inter. J. Syst. Bacterial. - 1999. - Vol. 49. - P. 803 - 813.
12. Impact of latent infections with Chlamydophila species in young cattle / P. Reinhold, J. Jaeger, E. Liebler-Tenorio [et al.] // Vet. J. - 2008. - Vol. 175. - P. 202 - 211.
13. High Prevalence of Natural Chlamydophila Species Infection in Calves / JunBae Jee, Fred J. Degraves, TeaYoun Kim [et al.] // J. Clin. Microbiol. - 2004. - Vol. 42, N. 12. - P. 5664 - 5672.
14. Levingstone, M. What is the prevalence and economic of chlamydial infections in cattle? The need to validate and harmonise existing methods of detection / M. Levingstone, D. Longbottom // Vet. J. - 2006. - Vol. 172. - P. 3 - 5.
15. Longbottom D. Animal Chlamydioses and Zoonotic Implications / D. Longbottom, L. J. Coulter // J. Comp. Path. - 2003. Vol. 128. - P. 217-244.
16. OmpA and antigenic diversity of bovine Chlamydophila pecorum strains / B. Kaltenboeck, E. Heinen, R. Schneider [et al.] // Vet. Microbiol. - 2008.
17. PCR Detection and Molecular Identification of Chlamydiaceae Species / J.C. Hartley, S. Kaye, S. Stivenson [et al.] // J. Clin. Microbiol. - 2001. - Vol. 39, N.9.- P: 3072 - 3079.
18. Philips, H. L. Spontaneous Change from Overt to Covert Infection of Chlamydia pecorum in Cycloheximide- Treated Mouse McCoy Cells / H. L. Philips, M. J. Clarkson // Infection and immunity. - 1995. Vol. 63, N 9. - P. 3729-3730.
19. Species Identification of Chlamydia Isolates by Analyzing Restriction Fragment Length Polymorphism of the 16S-23S rRNA Spacer Region / A. Meijer, G. J. Kwakkel, A. De Vries, [et al.] // J. Clin. Microbiol. - 1997. - Vol. 35, N. 5. - P. 1179 - 1183.
20. Basic Local Alignment Search Tool (BLAST). - Режим доступа: http://blast.ncbi.nlm.nih.gov/Blast.cgi
21. National Center for Biotechnology Information. - Режим доступа: http://www.ncbi.nlm.nih.gov
22. Официальный сайт федеральной службы по ветеринарному и фитосанитарному надзору. - Режим доступа: http://fsvps.ru
23. Chlamydia professional. Comprehensive. Evidence based. Authoritative. The comprehensive reference and education site to Chlamydia and the chlamydiae. - Режим доступа. http://www.chlamydiae.com
Рецензия
Для цитирования:
Шибаев М.А., Дудников С.А., Прохватилова Л.Б. ВЫЯВЛЕНИЕ CHLAMYDOPHILA PECORUM С ПОМОЩЬЮ ПОЛИМЕРАЗНОЙ ЦЕПНОЙ РЕАКЦИИ: ГЕНЕТИЧЕСКОЕ РАЗНООБРАЗИЕ И РАСПРОСТРАНЕНИЕ. Ветеринарная патология. 2009;30(3):56-63.