IMMUNOLOGICAL CHARACTERISTICS OF THE EXPERIMENTAL INACTIVATED VACCINE AGAINST INFLUENZA A SUBTYPE H5
https://doi.org/10.25690/VETPAT.2022.77.54.008
Abstract
The indicators reflecting the immunogenicity of the experimental avian influenza vaccine (H5) inactivated by emulsion «AviFluVak» produced by FGBI «VNIIZH», tested in 1/5 and whole doses were studied. Assessment of the post-vaccination titer of antibodies to the avian influenza virus in RTGA with the antigen of the «Yamal» strain after 21 days after vaccination were 7.2 ± 0.2 and 8.9 ± 0.3 (log₂), accordingly to the tested doses. The protective coeficients obtained in the acute experiment for both tested doses of the vaccine had the values Pr = 1 (Pr = 1 - Ef/n, where: Ef is the number of birds showing clinical signs of illness or death; n is the number of birds in this group (n = 12)). After artificial infection, according to PCR data, oral excretion of the virus lasted up to 5 days and stopped for 7 days (predicted titer 2 lg EID50/0.1cm3). The coeficient of increase in the titer of antibodies in the acute experiment after 14 days was not statistically significant. The vaccine «AviFluVac» was studied in parallel with the commercial vaccine «FluProtect H5», which was considered as a positive control. According to the totality of the studied indicators, the vaccine «AviFluVac» surpassed the commercial analogue.
About the Authors
N. V. Moroz
«ARRIAH»
Russian Federation
Russian Federation
S. V. Frolov
«ARRIAH»
Russian Federation
Russian Federation
L. O. Shcherbakova
«ARRIAH»
Russian Federation
Russian Federation
V. Y. Kulakov
«ARRIAH»
Russian Federation
Russian Federation
References
1. OIE. High Pathogenicity Avian Influenza (HPAI) - Situation report 11/04/2022. https://www.woah.org/app/uploads/2022/04/hpai-situation-report-20220411.pdf
2. Nicita Alessandro. (2008). Avian influenza and the poultry trade / Nicita Alessandro // The World Bank, Policy Research Working Paper Series. https://www.researchgate.net/publication/23550548
3. Peyre M. Avian influenza vaccines: a practical review in relation to their application in the field with a focus on the Asian experience / M. Peyre, G. Fusheng, S. Desvaux, F. Roger // Epidemiol. Infect. - 2009 Jan;137(1):1-21. doi: 10.1017/S0950268808001039. Epub 2008 Aug 14. PMID: 18700992 https://agritrop.cirad.fr/546006/1/document_546006.pdf
4. OIE. Terrestrial Animal Health 2021. Сhapter 3.3.4. Avian influenza (including infection with high pathogenicity avian influenza viruses), - P. 1-24. https://www.woah.org/fileadmin/Home/fr/Health_standards/tahm/3.03.04_AI.pdf
5. Katz J. M. Pathogenesis of and immunity to avian influenza A H5 viruses /j. M. Katz [et al.] // Biomedicine & Pharmacotherapy 2000; 54: 178-187. https://pubmed.ncbi.nlm.nih.gov/10872716/
6. Van der Goot J. A. Quantification of the effect of vaccination on transmission of avian influenza (H7N7) in chickens /j. A. Van der Goot [et al.] // Proceedings of the National Academy of Sciences USA 2005; 102: 18141-18146 https://pubmed.ncbi.nlm.nih.gov/16330777/
7. Swayne D. E. Inactivated North American and European H5N2 avian influenza virus vaccines protect chickens from Asian H5N1 high pathogenicity avian influenza virus / D. E. Swayne, C. W. Lee, E. Spackman // Avian Pathology2006; 35: 141-146. https://www.tandfonline.com/doi/full/ 10.1080/03079450600597956
8. Bublot M. Efficacy of a fowlpox-vectored avian influenza H5 vaccine against Asian H5N1 highly pathogenic avian influenza virus challenge / M. Bublot [et al.] // Avian Diseases 2007; 51: 498-500. https://bioone.org/journals/avian-diseases/volume-51/issue-s1/7624-042706R.1/Efficacy-of-a-Fowlpox-Vectored-Avian-Influenza-H5-Vaccine-Against/10.1637/7624-042706R.1.short
9. Kumar M. Association of serologic and protective responses of avian influenza vaccines in chickens / M. Kumar [et al.] // Avian Diseases 2007; 51: 481-483. https://pubmed.ncbi.nlm.nih.gov/17494614/
Review
For citations:
Moroz N.V., Frolov S.V., Shcherbakova L.O., Kulakov V.Y. IMMUNOLOGICAL CHARACTERISTICS OF THE EXPERIMENTAL INACTIVATED VACCINE AGAINST INFLUENZA A SUBTYPE H5. Russian Journal of Veterinary Pathology. 2022;(3):5-13. (In Russ.) https://doi.org/10.25690/VETPAT.2022.77.54.008
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