СОЗДАНИЕ КРИОБАНКА КЛЕТОК И ЛИЧИНОК МОРСКИХ БЕСПОЗВОНОЧНЫХ
Аннотация
Development of cryopreservation methods permits to remove of seasonal and geographic limitations in investigations
on sea animals, helps to establish cryobanks of rare and disappearing species. This work deals
with analysis of known cryoprotectors and the search for new substances with potential cryoprotective properties
for marine invertebrates. We studied a combined effect of the cryoprotectants both of carbohydrate
and lipid origin at the presence DMSO on cell viability and the RNA synthesis of frozen-thaw embryonic
mollusc and echinoderm cells. Cryoprotective properties of exogenous lipids correlated with their thermotropic
behavior and depended of both membrane stabilizers and bioantioxidants. These methods would be
a useful tool in cell technology of marine bioactive materials.
on sea animals, helps to establish cryobanks of rare and disappearing species. This work deals
with analysis of known cryoprotectors and the search for new substances with potential cryoprotective properties
for marine invertebrates. We studied a combined effect of the cryoprotectants both of carbohydrate
and lipid origin at the presence DMSO on cell viability and the RNA synthesis of frozen-thaw embryonic
mollusc and echinoderm cells. Cryoprotective properties of exogenous lipids correlated with their thermotropic
behavior and depended of both membrane stabilizers and bioantioxidants. These methods would be
a useful tool in cell technology of marine bioactive materials.
Об авторах
Н. А. Одинцова
Институт биологии моря Дальневосточного отделения РАН
Россия
Россия
А. В. Борода
Россия
Н. М. Санина
Россия
Э. Я. Костецкий
Россия
Список литературы
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2. Lane M, Maybach JM, Gardner DK. Addition of ascorbate during cryopreservation stimulates subsequent embryo development. 2002. Human Reproduction 17 (10): 2686-2693.
3. Naidenko TKh. Cryopreservation of Crassostrea gigas oocytes, embryos and larvae using antioxidant echinochrome A and antifreeze protein AFP1. 1997. Cryo-Letters 18: 375-382.
4. Odintsova NA, Kiselev KV, Sanina NM, Kostetsky EY. Cryopreservation of primary cell cultures of marine invertebrates. 2001. Cryo-Letters 22: 299- 310.
5. Odintsova NA, Ageenko NV, Kiselev KV, Sanina NM, Kostetsky EY. Analysis of marine hydrobiont lipid extracts as possible cryoprotective agents. Int. J. Refrigeration, 2006. 29: 387-395.
6. Sanina NM, Kostetsky EY. Thermotropic behavior of major phospholipids from marine invertebrates: changes with warm acclimation and seasonal acclimatization. Comp. Biochem. Physiol. 2002. 133B: 143-153.
7. Sanina NM, Goncharova SN, Kostetsky EY. Fatty acid composition of individual polar lipid classes from marine macrophytes. 2004. Phytochemistry 65: 721-730.
8. Tervit HR, Adams SL, Roberts RD, McGowan LT, Pugh PA, Smith JF, Janke AR. Successful cryopreservation of Pacific oyster (Crassostrea gigas) oocytes. Cryobiology. 2005. 51(2): 142-51.
Рецензия
Для цитирования:
Одинцова Н.А., Борода А.В., Санина Н.М., Костецкий Э.Я. СОЗДАНИЕ КРИОБАНКА КЛЕТОК И ЛИЧИНОК МОРСКИХ БЕСПОЗВОНОЧНЫХ. Ветеринарная патология. 2007;(3):254-256.
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