РАЗРАБОТКА НЕПРЯМОГО ВАРИАНТА ИММУНОФЕРМЕНТНОГО АНАЛИЗА ДЛЯ ОПРЕДЕЛЕНИЯ АНТИТЕЛ К РОТАВИРУСУ В СЫВОРОТКАХ КРОВИ КРС МЕТОДОМ ОДНОГО РАЗВЕДЕНИЯ
Abstract
The indirect ELISA for quantitative evaluation of antibodies against rotavirus in bovine blood sera using a
method of single dilution was developed. The equation of a standard curve obtained by the method of linear
regression was used for the prediction of titers using S/P ratio measured in a single dilution of serum. The
intervals of permissible values of optical density of control negative and positive sera as well as a positivenegative
threshold were determined. The specificity and sensitivity of the method were compared with
those of the commercial kit INGEZIM ROTAVIRUS (Ingenasa, Spain). The developed test-system was
used for the investigation of sera received from different holdings of the RF.
method of single dilution was developed. The equation of a standard curve obtained by the method of linear
regression was used for the prediction of titers using S/P ratio measured in a single dilution of serum. The
intervals of permissible values of optical density of control negative and positive sera as well as a positivenegative
threshold were determined. The specificity and sensitivity of the method were compared with
those of the commercial kit INGEZIM ROTAVIRUS (Ingenasa, Spain). The developed test-system was
used for the investigation of sera received from different holdings of the RF.
About the Authors
Г. СкитовичRussian Federation
О. Бьядовская
Russian Federation
Л. Прохватилова
Russian Federation
References
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Review
For citations:
, , . Russian Journal of Veterinary Pathology. 2007;(4):45-48. (In Russ.)
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