РАЗРАБОТКА МЕТОДИК ИНДИКАЦИИ И ИДЕНТИФИКАЦИИ ВОЗБУДИТЕЛЕЙ ИНФЕКЦИОННЫХ ЗАБОЛЕВАНИЙ ЖИВОТНЫХ ВИРУСНОЙ ЭТИОЛОГИИ НА ОСНОВЕ ПЦР-АНАЛИЗА
Abstract
The paper is devoted to the basic principles of viral genetic determinants study. Algorithms for target genes
selection, algorithms for primer pairs search using bioanalytical programmes, analysis of their quality, theoretical
and practical review of their matrix complementary and intraspecies specificity are described in the
paper. Besides, steps for optimization PCR procedures and verification of its quality parameters were demonstrated
using agents of herpesvirus disease as a pattern (infectious rhinotracheitis virus and avian infectious
laryngotracheitis virus).
selection, algorithms for primer pairs search using bioanalytical programmes, analysis of their quality, theoretical
and practical review of their matrix complementary and intraspecies specificity are described in the
paper. Besides, steps for optimization PCR procedures and verification of its quality parameters were demonstrated
using agents of herpesvirus disease as a pattern (infectious rhinotracheitis virus and avian infectious
laryngotracheitis virus).
About the Author
А. Герилович
Национальный научный центр «Институт экспериментальной и
клинической ветеринарной медицины», Харьков, Украина
Russian Federation
Russian Federation
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Review
For citations:
. Russian Journal of Veterinary Pathology. 2007;(4):107-110. (In Russ.)
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