МНОГОСЛОЙНЫЙ МЕТОД КУЛЬТИВИРОВАНИЯ ВИРУСА ГЕРПЕСА ИНДЕЕК
Abstract
In this paper the possibility of obtaining the maximum number of virus-containing cells infected by the turkey
herpes virus of strain Vladimir was studied using the method of multilayer cultivation in roller bottles.
It was determined that after adding of freshly-trypsinized cells to the infective maintenance medium
at the concentration of 0,6 (group I) and 1,2 mln cells/cm3 (group II), the final concentration of virus-containing
cells increased as compared with the control (9,8 mln cells/cm3) up to 13,6 and 17,1 mln cells/cm3,
respectively. Furthermore, the infectious activity of the virus increased as compared with the control value
(1,5060,09х106 FFU/cm3) up to 2,0260,082 (group I) and 2,8080,122х106 (group II) FFU/cm3. Thus, the
developed method of multilayer cultivation enables to improve the production technology of liquid virus
vaccines against Mareks disease.
herpes virus of strain Vladimir was studied using the method of multilayer cultivation in roller bottles.
It was determined that after adding of freshly-trypsinized cells to the infective maintenance medium
at the concentration of 0,6 (group I) and 1,2 mln cells/cm3 (group II), the final concentration of virus-containing
cells increased as compared with the control (9,8 mln cells/cm3) up to 13,6 and 17,1 mln cells/cm3,
respectively. Furthermore, the infectious activity of the virus increased as compared with the control value
(1,5060,09х106 FFU/cm3) up to 2,0260,082 (group I) and 2,8080,122х106 (group II) FFU/cm3. Thus, the
developed method of multilayer cultivation enables to improve the production technology of liquid virus
vaccines against Mareks disease.
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Review
For citations:
, . Russian Journal of Veterinary Pathology. 2007;(4):167-170. (In Russ.)
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