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ОЦЕНКА ИММУНОБИОЛОГИЧЕСКИХ СВОЙСТВ ИЗОЛЯТОВ MYCOPLASMA GALLISEPTICUM С ИСПОЛЬЗОВАНИЕМ ТРАХЕАЛЬНЫХ ОРГАННЫХ КУЛЬТУР

Abstract

The opportunity of using tracheal organ cultures for studying immunobiological properties of mycoplasma
is demonstrated in the paper. Optimal conditions for studies were selected. The conducted studies aimed at
the determination of the titer of pathogenic isolates of mycoplasma using tracheal organ cultures and evaluation
of their concentration in the colony-forming units showed comparable results. The results of comparative
evaluation of pathogenicity of field isolates and of strain R M. gallisepticum for chicks by determination
of ciliary activity of trachea epithelium are comparable with the results obtained by sequencing.

About the Authors

И. Рунина

Russian Federation


О. Чупина

Russian Federation


М. Сорокина

Russian Federation


Д. Андрейчук

Russian Federation


А. Спрыгин

Russian Federation


Н. Мудрак

Russian Federation


В. Дрыгин

Russian Federation


В. Ирза

Russian Federation


References

1. Подбор условий для выделения полевых изоля- тов возбудителей микоплазмозов птиц от боль- ных птиц и из проб патологического материала / И.А, Рунина, М.И. Сорокина, М.Ю. Волков [и др.] // 2-й Междунар. вет. конгр. по птицеводству. М., 2006. С. 89-92

2. Методические указания по приготовлению и под- держанию трахеальной органной культуры ку- риных эмбрионов и цыплят / О.А. Чупина [и др.] Владимир, 2004.

3. Bearson, S.M. Induction of a Mycoplasma gallisepticum pMGA gene in the chicken tracheal ring organ culture model / S.M. Bearson, S.D. Collier, B.L. Bearson // Avian Dis. 2003. Vol. 3. P. 745-749.

4. Cassell, G. H. Mycoplasmas respiratory infections / G.H. Cassell, W.A. Clyde, J.K. Davis // The Mycoplasmas. Mycoplasma Pathogenicity. Inc. Orlando, Acad. Press, 1985. Vol. 4. P. 69-106.

5. Feberwee, A. An experimental model to quantify horizontal transmission of Mycoplasma gallisepticum / A. Feberwee, D.R. Mekkes, D. Klinkenberg // Avian Pathol. 2005. Vol. 3. P. 355-361.

6. Papazisi, L. Analysis of cytadherence-deficient, GapA negative Mycoplasma gallisepticum strain R / L. Papazisi, K.E. Troy, T.S. Gorton // Infect. Immun. 2000. Vol. 68, №12. P. 6643-6649.


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 ,  ,  ,  ,  ,  ,  ,   . Russian Journal of Veterinary Pathology. 2007;(4):171-174. (In Russ.)

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ISSN 2949-4826 (Online)