ВЛИЯНИЕ ОХЛАЖДЕНИЯ И ЗАМОРАЖИВАНИЯ- ОТТАИВАНИЯ НА ТРАНСПОРТ ЭЛЕКТРОНОВ В ДЫХАТЕЛЬНОЙ ЦЕПИ МИТОХОНДРИЙ СПЕРМАТОЗОИДОВ
Abstract
Redox-reactions of NAD.H and FAD of boar and cock sperm during diluting, cooling and freezing - thawing
were studied. 2,4-dinitrophenol (DNP) and antimycin were used as testing substances. It was established,
that cold shock of undiluted sperm results in great reduction of NAD.H and FAD amount.
The growth of oxidation of NAD and FAD sperm after freezing - thawing was observed. Reduction of
reaction on injecting 2,4-DNP and antimycin was as well. The results of estimation of a redox-condition
NAD and FAD provided by a fluorescent method well correlated with data of polarographic estimation of
spermatozoa respiration after cooling and freezing - thawing.
were studied. 2,4-dinitrophenol (DNP) and antimycin were used as testing substances. It was established,
that cold shock of undiluted sperm results in great reduction of NAD.H and FAD amount.
The growth of oxidation of NAD and FAD sperm after freezing - thawing was observed. Reduction of
reaction on injecting 2,4-DNP and antimycin was as well. The results of estimation of a redox-condition
NAD and FAD provided by a fluorescent method well correlated with data of polarographic estimation of
spermatozoa respiration after cooling and freezing - thawing.
About the Authors
И. Шапиев
ВНИИ генетики и разведения сельскохозяйственных животных
Russian Federation
Russian Federation
Е. Никиткина
Russian Federation
Review
For citations:
, . Russian Journal of Veterinary Pathology. 2007;(4):227-229. (In Russ.)
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