РАЗРАБОТКА НЕПРЯМОГО ВАРИАНТА ИФА НА ОСНОВЕ РЕКОМБИНАНТНОГО БЕЛКА P65 ДЛЯ ОПРЕДЕЛЕНИЯ АНТИТЕЛ К MYCOPLASMA HYOPNEUMONIAE
Abstract
Mycoplasma hyopneumoniae recombinant p65 protein was prepared by expression in E. coli. Indirect enzyme
immunoassay has been developed on the basis of recombinant antigen, for the detection of antibodies
to M. hyopneumoniae in porcine blood sera. Very good consistency (k=0.97) has been established between
the developed test-system and commercial test kit Mycoplasma hyopneumoniae Antibody Test Kit
(IDEXX, USA).
immunoassay has been developed on the basis of recombinant antigen, for the detection of antibodies
to M. hyopneumoniae in porcine blood sera. Very good consistency (k=0.97) has been established between
the developed test-system and commercial test kit Mycoplasma hyopneumoniae Antibody Test Kit
(IDEXX, USA).
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Review
For citations:
, . Russian Journal of Veterinary Pathology. 2006;(4):109-113. (In Russ.)
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