BIOLOGICAL PROPERTIES OF THE INFECTIOUS BURSAL DISEASE VIRUS ISOLATES IDENTIFIED IN RUSSIAN FEDERATION IN 2017-2019
https://doi.org/10.25690/VETPAT.2020.46.82.007
Abstract
The objective of the present research was to study the genome structure and the degree of pathogenic action (virulence) of five IBD virus isolates (Z/Lub, G/Mar, B/VN, K/Pol and S/Agr) isolated at poultry in the farms of Russian Federation. The structure of the VP2 fragment was examined. Strain «BG» with the known structure of this fragment was used as a control. The amino acid sequence alignment showed that the isolates G/Mar, Z/Lub, and S/Agr had a high degree of homology with the control strain, but difiered from the control at position D279N (aspartic acid residue). The B/VN and K/Pol isolates in this position were not identical to the three materials indicated. The virulence of the isolates was assessed in terms of morbidity, mortality, and bursa atrophy. The known virulent strain «52/70» was used as a control. It was found that in relation to the control strain, the isolate G/Mar had the highest estimate of virulence (80.5 %). The next were isolates Z/Lub (55.4 %) and S/Agr (51.4 %). The least virulent isolates were B/VN (44.3 %) and K/Pol (43.9 %). Note that the three most virulent isolates were identical at position D279N. The results obtained indicate the possibility of a certain relationship between the structure of the genome and the phenotype of the virus. time
About the Authors
T. N. Zybina
«All-Russian scientific research Institute for the Protection of Animals»
Russian Federation
Russian Federation
A. A. Pyatkina
«All-Russian scientific research Institute for the Protection of Animals»
Russian Federation
Russian Federation
N. V. Moroz
«All-Russian scientific research Institute for the Protection of Animals»
Russian Federation
Russian Federation
V. Y. Kulakov
«All-Russian scientific research Institute for the Protection of Animals»
Russian Federation
Russian Federation
L. O. Hcherbakova
«All-Russian scientific research Institute for the Protection of Animals»
Russian Federation
Russian Federation
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Review
For citations:
Zybina T.N., Pyatkina A.A., Moroz N.V., Kulakov V.Y., Hcherbakova L.O. BIOLOGICAL PROPERTIES OF THE INFECTIOUS BURSAL DISEASE VIRUS ISOLATES IDENTIFIED IN RUSSIAN FEDERATION IN 2017-2019. Russian Journal of Veterinary Pathology. 2020;(3):24-31. (In Russ.) https://doi.org/10.25690/VETPAT.2020.46.82.007
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