BIOCHEMICAL PROPERTIES OF MICROORGANISMS TREATED WITH IVERTIN

Abstract

Prescribed anthelminthic preparations may trigger infection process in animals. In this respect, it is necessary to study variability of biological properties of microorganisms when treated with anthelminthic preparations, Ivertin, in particular, at chroboliological approach. Research into chronobiological rhythms is interesting in the way of determining ecological and biological properties of pathogenic and potentially pathogenic microorganisms. The study involved several control measurements in 24 hours with 3 - 5 replicas of experimental conditions. Growth pattern in the studied cultures treated with anthelmintic preparation was studied on the plain broth with addition of 1% of Ivertin. The control was the cultures grown without test solution adding. Introduction of anthelmintic Ivertin into nutrient media of growing microorganisms returned in considerable changes in their biological properties. We have determined the following chronobiological pattern: the highest Ivertindriven rate of difference in ultradian rhythm compared to the control culture, was found, in streptococci (63%), with slightly lower rate in listeria (54%). In the daily rhythm, i.e. circadian, both L.monocytogenes and Str.pneumoniae demonstrated 54% difference rate compared to the control Str.pneumoniae. In the infradian rhythm, which is more than 24 hours, the highest variability was detected in St. aureus culture (72%). Therefore, experiment of growing microbial cultures in presence of Ivertin anthelmintic demonstrated the preparation’s active effect on enzymatic activity of the studied strains compared to control which revealed through lower activity of saccharolytic and proteolytic enzymes.