METHODOLOGY FOR IDENTIFYING PASTEURELLA MULTOCIDA IN THE BLOOD OF INFECTED AND DEAD BIRDS

The aim of research was to improve the methodology of identification of Pasteurella multocida in the blood of infected and dead birds on the basis of microscopy. In numerous experiments have used 11 strains of the most common among birds. There were serovar A:1 P. multocida: X-73 (from the collection of Heddlestouna, USA), control strains: 55, 115, 712, 915, 1931 (VGNKI veterinary products) and 5 virulent natural ones. Morphogenesis of bacteria in the organism and poultry carcass has studied depending on the route of contamination, the contaminating material, the temperature and the liquid phase environment. The research results served as the basis of significantly changing of traditional idea of the morphology of P. multocida cells. It is shown at the level of light and electron microscopies (including the formation of the capsule and bipolarity) P. multocida shaping in the dynamics in the body and the corpse. For the first time found conditions for the formation of the capsule, the explanation of the phenomenon of bipolarity and of explaining the functional morphology and thermolability of P. multocida. It is shown that temperature is a factor of influence on P. multocida capsule forming, changes the morphology of the bacteria and leads to its penetration into the body of a bird. Compared with the traditional options presented methodology is distinguished by objective research, the reliability of the result, minimizing material costs and labor time. The aim of research was to improve the methodology of identification of Pasteurella multocida in the blood of infected and dead birds on the basis of microscopy. In numerous experiments have used 11 strains of the most common among birds. There were serovar A:1 P. multocida: X-73 (from the collection of Heddlestouna, USA), control strains: 55, 115, 712, 915, 1931 (VGNKI veterinary products) and 5 virulent natural ones. Morphogenesis of bacteria in the organism and poultry carcass has studied depending on the route of contamination, the contaminating material, the temperature and the liquid phase environment. The research results served as the basis of significantly changing of traditional idea of the morphology of P. multocida cells. It is shown at the level of light and electron microscopies (including the formation of the capsule and bipolarity) P. multocida shaping in the dynamics in the body and the corpse. For the first time found conditions for the formation of the capsule, the explanation of the phenomenon of bipolarity and of explaining the functional morphology and thermolability of P. multocida. It is shown that temperature is a factor of influence on P. multocida capsule forming, changes the morphology of the bacteria and leads to its penetration into the body of a bird. Compared with the traditional options presented methodology is distinguished by objective research, the reliability of the result, minimizing material costs and labor time.

пастереллёз птиц, путь заражения, температурный фактор, морфогенез P. multocida, капсула, биполярность, методология выявления

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