ГЕНОТИПИРОВАНИЕ РАЗЛИЧНЫХ ШТАММОВ CLOSTRIDIUM PERFRINGENS ВЫДЕЛЕННЫХ ОТ СУК С НЕКРОЗОМ БЕРЕМЕННОЙ МАТКИ

Abstract

A polymerase chain reaction protocol consisting of a multiplex to identify the cpa, cpbl, cpetx, cpi genes and a duplex to identify the cpe and cpb2 genes encoding for a, .1, ., . enterotoxin and .2 toxins, respectively, was applied to DNA extracted from four collections of Clostridium peifringens strains. Strains of the first collection were isolated from the feces of 43 healthy dogs. The second collection was obtained from the feces of 27 dogs with various lesions of the gastrointestinal tract. Third collection of isolates were obtained at autopsy from the bodies of seven dogs, and finally, the fourth collection of isolates obtained from the cavity of the uterus removed 12 dogs diagnosed with - «necrosis of the pregnant uterus.» Cpa gene responsible for synthesis of the toxin ., was detected in all the investigated samples of DNA. Genes cpb1, cpetx, cpi were not detected, and all isolates were identified as toksinotip A. The gene cpa, cpb2 gene, together with (the genotype of cpa + cpb2), was detected in 22 samples of DNA isolates of C. perfringens isolated from the feces of healthy dogs (51.2%), 5 isolates of DNA samples isolated from the feces of dogs with enteropathies (71.5%). However, in samples of DNA isolates obtained from the contents of necrotic uterus cpa + cpb2 genotype found only in one dog (8.3%) and in samples obtained during autopsies of dogs only in 5 out of 27 cases (18.5%).