THE USE OF THE CONTINUOUS CALF KIDNEY CELLS CULTURE TAURUS-1 (T-1) FOR THE CULTIVATION OF BOVINE ADENOVIRUSES

The article presents the results of a study of the cultural properties of bovine adenoviruses: Bovine-10 (serotype 1), Bovine-19 (serotype 2), WBR-1 (serotype 3), THT-62 (serotype 4), B/4 65 (serotype-5), 671/130 (serotype 6), Fucuroi (serotype 7), Misk (serotype 8), Sofa 4/67 (serotype 9), Standel (serotype 10), CLB isolates and Rus 18/81 identified as the first and seventh serotype. Adenoviruses with 1-10 serotypes were cultured in continuous cell cultures of PT-80 (calf kidney), MDBC (cow kidney), TR (cow trachea), Taurus-1(T-1) (calf kidney - fibroblast-like clone) cells for 15 passages, as well as in primary culture of TB cells (bull testicles). The culture medium MEM with a double set of amino acids with the addition of 10 % of fetal bovine blood serum was used as a nutrient medium. The cell monolayer was grown in a CO2 incubator at a temperature of 37 °C. Virus titers were taken into account after the 15th passage, which ranged from 5.0 to 8.86 lg TCD50/ml. The titer of infectious activity was determined according to the method of Reed and Mench, expressed in lg TCD50/ml

continuous cell cultures: PT-80, MDBC, TR, Taurus-1 (T-1), primary cell culture: TB, LEK, PEK, adenoviruses of 1-10 serotypes, isolates of adenoviruses of KLB and Rus 18/81

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