BIOLOGICAL PROPERTIES OF THE RABBITS MYXOMATOSIS VIRUS STRAIN «Mixo/ARRIAH-18»
https://doi.org/10.25690/VETPAT.2022.44.27.004
Abstract
We studied the biological properties of the myxomatosis virus Mixo/ARRIAH-18 strain in cultivated on a transplantable RK-13. The cell culture was grown as a monolayer on tissue culture flasks. The aim of the research was to standardize the conditions for obtaining the virus and evaluate its immunological effect on the animals. According to the known number of cells that make up the monolayer in the mattress (E = 1.885 × 10⁶), the volume of the nutrient medium (V, cm³), the given doses of the virus, TCI₅₀ (D) and the multiplicity of infection per cell (C = D/E, TCI₅₀/cell. ), we determined the indicators characterizing the reproduction of the virus in this culture: the time of development of CPE ≥ 75 % (L, hours); infectious titer in culture fluid, TCD₅₀/cm³ (T); virus reproduction coeffcient (lg K = lg T + lg V - lg E, lg TCD₅₀/cell). As a result of the studies, it was found that effective reproduction of the vaccine virus in the RK-13 cell culture will occur at a multiplicity of infection of at least 0.017 TCD₅₀/cell. In this case, the defeat of at least 75 % of the cells should be detected on average within no more than 19.33 hours, which will lead to the accumulation of the pathogen in the culture fluid in a titer of at least 6.33 lg TCD₅₀/cm³. As experimental animals, rabbits of 45 days of age, not immune to myxomatosis, were used. The immunological effect of vaccinating rabbits with the studied strain, the assessment of which corresponds to the neutralization index adopted in GOST 28086 (lg IN = 3.0), was achieved using an immunizing dose of D ≈ 4.0 lg TCD₅₀. According to the results of IFA, the neutralization index lg IN = 3.0 corresponded to the assessment of the average logarithmic titer of antibodies lg Ts = 3.138 ± 0.105 or, according to the lower limit of the confidence interval (p ≈ 0.05), lg Ts - 2S = 3.138 - 0.210 = 2.928, which corresponds to the value Ts = 847.
About the Authors
E. D. Kunikova
«ARRIAH»
Russian Federation
Russian Federation
N. V. Moroz
«ARRIAH»
Russian Federation
Russian Federation
A. A. Pyatkina
«ARRIAH»
Russian Federation
Russian Federation
V. Y. Kulakov
«ARRIAH»
Russian Federation
Russian Federation
References
1. Демидова Т. Н. Прижизненное и посмертное проявление миксоматоза кроликов в условиях Нижегородской области / Т. Н. Демидова, Ю. В. Пашкина // Вопросы нормативно-правого регулирования в ветеринарии. - 2014. - № 2. - С. 74-76
2. Коломыцев А. А. Миксоматоз кроликов // А. А. Коломыцев // Ветеринария сельскохозяйственных животных. - 2008. - № 2. - С. 9-13
3. Коломыцев А. А. Миксоматоз кроликов / А. А. Коломыцев, О. Н. Бурдинская // Кролиководство и звероводство. - 2005. - № 6. - С. 22-26
4. Шевченко А. А. Болезни кроликов / А. А. Шевченко, Л. А. Шевченко, А. М. Литвинов / - М.: «АКВАРИУМ БУК», 2002. - 224 с
5. Бабкин И. В. Временной масштаб эволюции поксвирусов / И. В. Бабкин, С. Н. Щелкунов // Молекулярная биология. - 2006. - Т. 40. - № 1. - С. 20-24
6. Fenner's Veterinary Virology / ed. N. Maclachlan, E. J. Dubovi. - 5th ed. - Academic Press. - 2016. - P. 157-174
7. International Committee on Taxonomy of Viruses ICTV. URL: https://talk.ictvonline.org/taxonomy/
8. Origin and Evolution of Viruses / ed. E. Domingo, C. R. Parrish, J. J. Holland. - 2nd ed. - Academic Press. - 2008. - P. 431-446
9. Россельхознадзор: Федеральная служба по по ветеринарному и фитосанитарному надзору / Эпизоотическая ситуация. URL: https://fsvps.gov.ru/fsvps/iac
10. Kerr P. J. Genomic and phenotypic characterization of myxoma virus from Great Britain reveals multiple evolutionary pathways distinct from those in Australia / P. J. Kerr, I. M. Cattadori, M. B. Rogers [et al.] // PLoS Pathogens. - 2017. - № 13 (3). - 23 p. URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349684/pdf/ppat.1006252.pdf
11. Manev I. Humoral immune response to different routes of myxomatosis vaccine application / I. Manev, K. Genova, A. Lavazza, L. Capucci // World Rabbit Science. - 2018. - Vol. 26. - № 2. - P. 149-154
12. Spiesschaert K. The status and future directions of myxoma virus, a master in immune evasion / B. Spiesschaert, G. McFadden, K. Hermans [et al.] // Veterinary Research. - 2011. - № 76. - 18 p. URL: https://veterinaryresearch.biomedcentral.com/track/pdf/10.1186/1297-9716-42-76
13. OIE Terrestial Manual 2021. Chapter 3.7.1. - 20 p. URL: https://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/3.07.01_MYXO.pdf
14. Культивирование клеток млекопитающих: учеб.-метод. пособие / сост. И. С. Глаголева, Э. Р. Булатов, Р. Г. Киямова. - Казань: Изд-во Казанского ун-та, 2019. - 44 с
15. Использование в вирусологии культуры клеток: метод. указания / сост. И. Я. Строганова, А. А. Трухоненко. - Красноярск: Изд-во Красноярского гос. аграр. ун-та, 2013. - 48 с
16. ГОСТ 28086. Вакцины против миксоматоза кроликов живые. Технические условия. - Введ. 01.01.2015. - М.: Стандартинформ, 2014. - 12 с
Review
For citations:
Kunikova E.D., Moroz N.V., Pyatkina A.A., Kulakov V.Y. BIOLOGICAL PROPERTIES OF THE RABBITS MYXOMATOSIS VIRUS STRAIN «Mixo/ARRIAH-18». Russian Journal of Veterinary Pathology. 2022;(1):48-55. (In Russ.) https://doi.org/10.25690/VETPAT.2022.44.27.004
Views:
65
Email this article 