ИЗУЧЕНИЕ БИОЛОГИЧЕСКИХ СВОЙСТВ ИЗОЛЯТА МЕТАПНЕВМОВИРУСА ПТИЦ aMPV/B/2-07
Abstract
Biological characteristics of avian metapneumovirus isolate aMPV/B/2-07, collected from chick-broilers in
2007, were studied. The virus was isolated in tracheal organ culture. The virus-containing material was administered
in chicks by intranasal, oral routes and into a conjunctival sac. Birds demonstrated respiratory
organ affection. The virus was detected in infected chicks by the RT-PCR in oral swabs in 2-7 days, in tissues
of nasal passages in 3, 6 days following infection. In in-contact non-affected chicks the virus was identified in
oral swabs in 4-10 days, in tissues of nasal passages and trachea in 3, 6, 10 days following infection.
2007, were studied. The virus was isolated in tracheal organ culture. The virus-containing material was administered
in chicks by intranasal, oral routes and into a conjunctival sac. Birds demonstrated respiratory
organ affection. The virus was detected in infected chicks by the RT-PCR in oral swabs in 2-7 days, in tissues
of nasal passages in 3, 6 days following infection. In in-contact non-affected chicks the virus was identified in
oral swabs in 4-10 days, in tissues of nasal passages and trachea in 3, 6, 10 days following infection.
About the Authors
З. ХлебовецRussian Federation
А. Меньщикова
Russian Federation
М. Качалова
Russian Federation
Л. Щербакова
Russian Federation
Н. Мудрак
Russian Federation
А. Борисов
Russian Federation
В. Дрыгин
Russian Federation
References
1. Условия получения трахеальной органной куль- туры куриных эмбрионов для выделения и типи- рования вируса инфекционного бронхита кур / О. А. Чупина, С. В. Фролов, В. И. Диев, А. В. Борисов // Сибирская язва и др. опасные инфекц. болезни жив-х: Матер. по теме работы круглого стола, приуроченного к 80-летию академика РАСХН Бакулова И. А. - Покров, 2005. - С. 225-227.
2. Antigenic differentiation of strains of turkey rhinotracheitis virus using monoclonal antibodies / J. K. A. Cook, B. V. Jones, M. M. Ellis [et al.] // Avian Pathol. 1993. Vol. 22. P. 257-273.
3. Avian pneumovirus infection in broiler chicks inoculated with Escherichia Coli at different time intervals / A. R. Al-Ankari, J. M. Bradbury, C. J. Naylor [et al.] // Avian Pathol. 2001. Vol. 30. P. 257-267
4. Avian pneumovirus infection in Minnesota turkeys: experimental reproduction of the disease / F. F. Jirjis, S. L. Noll, D. A. Halvorson [et al.] // Avian Dis. 2000. Vol. 44. P. 222-226
5. Cook, J. K. A. Avian rhinotracheitis / J. K. A. Cook // Rev. sci. tech. Off. int. Epiz. 2000. Vol. 19, № 2. P. 602- 613.
6. Cook, J. K. A. The pathogenesis of turkey rhinotracheitis virus in turkey poults inoculated with the virus alone or together with two strains of bacteria / J. K. A. Cook, M. M. Ellis, M. B. Huggins // Avian Pathol. 1991. Vol. 20. P. 155-166.
7. Exacerbation of Mycoplasma gallisepticum infection in turkeys by rhinotracheitis virus / C. J. Naylor, A. R. Al-Ankari, A. I. Al-Afaleq [et al.] // Avian Pathol. 1992. Vol. 21. P. 295-305.
8. Experimental and serologic observations on Avian pneumovirus (APV/turkey/Colorado/97) infection in turkeys / B. Panigrahy, D. A. Senne, J. C. Pedersen [et al.] // Avian Dis. 2000. Vol. 44. P. 17-22
9. Experimental infection in turkeys and chickens with Ornitobacterium rhinotracheale / P. V. Empel, H. V. D. Bosch, D. Goovaerts, P. Storm // Avian Dis. 1996. Vol. 40. P. 558-564
10. Experimental infections of turkeys, chickens, ducks, geese, guinea fowl, pheasants and pigeons with turkey rhinotracheitis virus / R. Gough, M. S. Collins, W. J. Cox, N. J. Chettle // Vet. Rec. 1988. Vol. 123. P. 58-59.
11. Isolation of a pneumovirus from a Muscovy duck / D. Toquin, M. H. Bayцn-Auboyer, N. Eterradossi [et al.] // Vet. Rec. 1999. Vol. 139. P. 71-72.
12. Jones, R. C. Avian pneumovirus infection: questions still unanswered / R. C. Jones // Avian Pathol. 1996. Vol. 25. P. 639-648.
13. Juhasz, K. Extensive sequence variation in the attachment (G) protein gene of avian pneumovirus: evidence for two distinct subgroups / K. Juhasz, A. J. Easton // J. Gen. Virol. 1994. Vol. 75. P. 2873-2880.
14. Longitudinal field studies of infectious bronchitis virus and avian pneumovirus in broilers using typespecific polymerase chain reactions / D. Cavanagh, K. Mawditt, P. Britton, C. J. Naylor // Avian Pathol. 1999. Vol. 28. P. 593-605.
15. Nucleotide sequences of the F, L and G protein genes of two non-A/non-B avian pneumoviruses (APV) reveal a novel APV subgroup / M. H. Bayцn- Auboyer, C. Arnauld, D. Toquin, N. Eterradossi // J. Gen. Virol. 2000. Vol. 81. P. 2723-2733
16. Pathogenesis of Avian pneumovirus infection in turkeys / F. F. Jirjis, S. L. Noll, D. A. Halvorson et al. // Vet. Pathol. 2002. Vol. 39. P. 300-310
17. Pathogenicity, transmissibility, and tissue distribution of Avian pneumovirus in turkey poults / A. N. Alkhalaf, L. A. Ward, R. N. Dearth, Y. M. Saif // Avian Dis. 2002. Vol. 46. P. 650-659.
18. Seal, B. Matrix protein gene nucleotide and predicted amino acid sequence demonstrate that the first US avian pneumovirus isolate is distinct from European strains / B. Seal // Virus Res. 1998. Vol. 58. P. 45-52.
19. The use of virus isolation, histopathology and immunoperoxidase techniques to study the dissemination of chicken isolate of avian pneumovirus in chickens / E. Catelli, J. K. A. Cook, J. Orbell [et al.] // Avian Pathol. 1998. Vol. 27. P. 632-640.
20. Turkey rhinotracheitis virus and Escherichia coli experimental infection in chickens: histopathological, immunocytochemical and microbiological study / N. Majo, X. Gibert, M. Vilafranca [et al.] // Vet. Microbiol. 1997. Vol. 57. P. 29-40.
Review
For citations:
, , , , , , . Russian Journal of Veterinary Pathology. 2007;(4):141-146. (In Russ.)
Views:
41
Email this article 