ЗАМОРАЖИВАНИЕ ЭМБРИОНОВ КРУПНОГО РОГАТОГО СКОТА ПОЛУЧЕННЫХ IN VITRO
Аннотация
Although frozen-thawed in vitro embryos have resulted in the birth of many thousand of normal healthy
calves, it is established that such embryos are much more sensitive to freezing than in vivo-derived embryos.
Pregnancy rates such embryos are generally lower than those reported for in vivo-derived. There are many
well-documented differences at the morphological, ultrastructural, metabolic, biochemical level between
the two categories of embryos. Chilling of in vitro derived bovine embryos depends on their developmental
stage, culture conditions and on presence of numerous lipid droplets in their cytoplasm
calves, it is established that such embryos are much more sensitive to freezing than in vivo-derived embryos.
Pregnancy rates such embryos are generally lower than those reported for in vivo-derived. There are many
well-documented differences at the morphological, ultrastructural, metabolic, biochemical level between
the two categories of embryos. Chilling of in vitro derived bovine embryos depends on their developmental
stage, culture conditions and on presence of numerous lipid droplets in their cytoplasm
Список литературы
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3. Pollard, J.W.; Leibo, S.P. Chiling sensitivity of mammalian embryos.Theriogenology. 1994. v. 41. 101-106.
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8. Rizos D. et.al. Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: implications for blastocyst yield and blastocyst quality. Mol. Reprod. Dev. 2002. 61. 234-248.
9. Rizos D. et. al. Analysis of differential messenger RNA expression between bovine blastocysts produced in different culture systems: implications for blastocyst quality. Biol Reprod 2002. 66. 589-95.
10. Gordon. I. Laboratory production of cattle embryos. 2003.
11. Imai K. et. al. Different factors affect developmental competence and cryotolerance in in vitro produced bovine embryos. 2002. 64 (10). 887-891.
12. Sang-Rae Cho. Enhanced Cryosurvival of Bovine Blastocysts Produced in Vitro in Serum-Free Medium Journal of Assisted Reproduction and Genetics. 2002 V. 19 (10). 487-492
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Рецензия
Для цитирования:
Сингина Г.Н., Эрнст Л.К., Тарадайник Т.Е. ЗАМОРАЖИВАНИЕ ЭМБРИОНОВ КРУПНОГО РОГАТОГО СКОТА ПОЛУЧЕННЫХ IN VITRO. Ветеринарная патология. 2007;(4):223-225.
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