РАЗРАБОТКА НЕПРЯМОГО ВАРИАНТА ИММУНОФЕРМЕНТНОГО АНАЛИЗА ДЛЯ ОПРЕДЕЛЕНИЯ ТИТРА АНТИТЕЛ К ВИРУСУ БОЛЕЗНИ АУЕСКИ В СЫВОРОТКАХ КРОВИ СВИНЕЙ
Abstract
A simple and sensitive ELISA for detection of antibody to Aujeszky`s disease virus in porcine sera was developed.
An inactivated concentrated cultural Aujezky`s disease virus («Arsky» strain), that was previously
treated by the NP-40 detergent, was used as an antigen. The results of comparative testing of porcine sera
using the suggested ELISA, microneutralization test and commercial CHEKIT-Aujeszky test-kit (Bommeli-
IDEXX) are presented in the paper. The relative specificity and sensitivity of the developed assay were
determined. The developed test-system was used for screening studies of the immune status of pigs on different
pig farms of the Russian Federation.
An inactivated concentrated cultural Aujezky`s disease virus («Arsky» strain), that was previously
treated by the NP-40 detergent, was used as an antigen. The results of comparative testing of porcine sera
using the suggested ELISA, microneutralization test and commercial CHEKIT-Aujeszky test-kit (Bommeli-
IDEXX) are presented in the paper. The relative specificity and sensitivity of the developed assay were
determined. The developed test-system was used for screening studies of the immune status of pigs on different
pig farms of the Russian Federation.
References
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2. Н.И. Закутский, И.Ю. Хухоров, В.И. Жестерев, и др. Герпесвирусные болезни животных. Владимир/Покров: Фолиант, 2003. 283 с.
3. Под ред. С. Херрингтона, Дж. Макги. Молекулярная клиническая диагностика. Методы: пер. с англ. М.: Мир, 1999. 558 с., ил.
Review
For citations:
. Russian Journal of Veterinary Pathology. 2006;(4):103-106. (In Russ.)
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