THELAZIOSIS IN WISENTS OF THE SHAHDAG NATIONAL PARK (AZERBAIJAN)

In the Shahdag National Park (Azerbaijan) the natural grouping of wisents (European bisons) is being formed. The program should eventually result in obtaining the independent stable grouping of wisents, capable of existing and reproduction without human interference. In the course of work with the grouping such a disease as wisent thelaziosis was detected. The presence of the disease can negatively affect the efficiency of the wisents reintroduction program. The aim of the work was to identify the problem, describe the clinical cases of wisent thelaziosis and determine possible measures to minimize risks for animals health. The article presents the data collected throughout a three years period of wisent group formation. The diagnosis – thelaziosis – was confirmed by finding the mature thelazia specimens of Thelazia rhodesi species. Such clinical manifestations as photophobia, eyelid edema, pathological lacrimation, serous conjunctivitis, purulent-catarrhal keratoconjunctivitis, clouding and ulceration of the cornea up to perforating ulcers, iridocyclitis were observed in wisents. The clinical form of thelaziosis had been detected in a wisent herd at all seasons of the year, at maximum in the period from the end of May to the end of July, up to 18.8 % of animals in the herd. At the rest of the time, the percentage of animals with disease manifestation varied from 3.7 to 11 %; two years in a row the cases of disease manifestation in adult wisents were observed in February. One case of thelaziosis was registered in a 1.5-month-old calf, the disease evolved in a hyperacute form. For treatment against thelaziosis in wisents the single intramuscular injections of ivermectin at a dose of 200 μg/kg (Ivermec, 2 ml/100 kg) were used. Medical treatment was given frontally to the herd after each detected clinical case. In addition wisents with strongly expressed clinical signs received a single injection of tulathromycin at a dose of 2.5 mg/1 kg (Draxin, 2.5 ml/100 kg). Medications were injected by means of a remote gun Dan-Inject. In Azerbaijan thelaziosis is spread among productive animals, as evidenced by veterinary reports. However, systematic studies of thelaziosis were carried out only in the 70–80s. As follows from the studies the thelaziosis infection rate in Azerbaijan has clearly expressed zonal distribution: high infection rate in the plain and submontane zones and rare cases of disease occurrence in the mountain belt. It was concluded that when forming wisents population, it is necessary to take into account the factor of zonality and plan the populations’ release in the mountainous areas of the national park, as the safest from veterinary side. Also due to domestic animals being the main source of infection for wisents in Azerbaijan, it is necessary to conduct research on disease expansion and intensity of infection in domestic livestock in the areas bordering with the Shahdag National Park. Currently while wisents are kept at the quarantine and transfer place, given the proximity of the enzootic focus, it is recommended to conduct the systematic frontal preventive treatment with ivermectin.

1. –6. Vide supra.

2. Al-Fatlawi M. A. A. Anisimova Dvukrylye nasekomye (Insecta: diptera) – perenoschiki vozbuditelej telyazioza krupnogo rogatogo skota v Srednem Mezhdurech’e (Irak) [Two-winged insects (Insecta: diptera) – carriers of causative agents of thelaziosis of cattle in the Middle Mesopotamia (Iraq)] / M. A. A. Al-Fatlawi, M. V. Volosach, S. V. Buga, E. I. Anisimova // Trudy BGU. – 2013. – Tom 8. – Chast’ 1. – S. 79–85.

3. Hristianovskij P. I. Telyaziozy krupnogo rogatogo skota v RF (retrospektiva i sovremennost’) [Bovine telazioses in the Russian Federation (retrospective and modernity)] / P. I. Hristianovskij, V. V. Belimenko, I. V. Zinin // RVZh, SHZh, – № 1/2014. – S. 36–38.

4. –11. Vide supra.

5. Krastin N. I. Rasshifrovka cikla razvitiya nematody Thelazia rhodezi (Desmarest, 1927), parazitiruyushchej v glazah krupnogo rogatogo skota [Deciphering the cycle of development of the nematode Thelazia rhodezi (Desmarest, 1927), parasitizing in the eyes of cattle] / N. I. Krastin // Dokl. AN SSSR. Nov. seriya. – 1949. – T. 64. – № 6. – S. 885–887.

6. Krastin N. I. Rasshifrovka biologicheskogo cikla vtorogo vozbuditelya telyazioza krupnogo rogatogo skota [Deciphering the biological cycle of the second causative agent of thelaziosis in cattle] / N. I. Krastin // Veterinariya. – 1950. – № 2. – S. 20–21.

7. Krastin N. I. Rasshifrovka cikla razvitiya nematody Th. skrjabini Erchow - 1928, parazita glaz krupnogo rogatogo skota [Deciphering the developmental cycle of the nematode Th. skrjabini Erchow - 1928, a parasite of the eyes of cattle] / N. I. Krastin // Doklad AN SSSR. – 1952. – T. 82. – № 5. – S. 829– 831.

8. –16. Vide supra.

9. Klesov M. D. Izuchenie biologii nematody Thelazia rhodesi Desm [Studying the biology of the nematode Thelazia rhodesi Desm] / M. D. Klesov // Zool. zhurnal. – 1949. – T. 28. – Vyp. 6. – S. 515–522.

10. Vide supra.

11. Krastin N. I. Telyaziozy zhivotnyh i ih vozbuditeli [Animal telazioses and their pathogens]: avtoref. diss. ... d-ra veterinar. nauk: 03.00.19 / Krastin Nikolaj Ivanovich. – M., 1953. – 21 s.

12. Krastin N. I. Izuchenie epizootologii telyazioza krupnogo rogatogo skota [Study of the epizootology of thelaziosis in cattle] / N. I. Krastin // Tr. Dal’nevostochnogo NIVI. – 1949. – T. 2. – S. 313–327.

13. –24. Vide supra.

14. Krastin N. I. Izuchenie epizootologii telyazioza glaz krupnogo rogatogo skota v Habarovskom krae [Study of the epizootology of telaziosis of the eyes of cattle in the Khabarovsk Territory] / N. I. Krastin, V. M. Ivashkin // Tr. Dal’nevostochnogo NIVI. – 1945. – T. 1. – S. 73–78.

15. Ivashkin V. M. Musca vitripennis promezhutochnyj hozyain Thelazia gulosa [Musca vitripennis intermediate host of Thelazia gulosa] / V. M. Ivashkin, G. Ya. Shmytova, M. G. Toktouchikova // Veterinariya. – 1966. – № 7. – S. 51–52.

16. Stepanov I. A. Promezhutochnye hozyaeva telyazij v usloviyah Mordovskoj ASSR [Intermediate hosts of telazia in the conditions of the Mordovian ASSR] / I. A. Stepanov // Uch. zap. / Mord. gos. unt. – 1963. – № 24. – C. 49–54.

17. –30. Vide supra.

18. Hromova L. A. Zarazhyonnost’ pastbishchnyh muh predgornogo Dagestana lichinkami nematod, parazitiruyushchimi u domashnih zhivotnyh [Infestation of pasture flies in foothill Dagestan with nematode larvae parasitizing domestic animals] / L. A. Hromova // Tr. Gel’mintologicheskaya laboratoriya AN SSSR. – 1979. – T. 29. – S. 171–176.

19. –33. Vide supra.

20. Napadovskaya T. V. Effektivnost’ sredstv patogeneticheskoj terapii v sochetanii s medikamentoznymi sredstvami pri kon”yunktivo-keratitah krupnogo rogatogo skota [The effectiveness of pathogenetic therapy in combination with medications for conjunctival keratitis in cattle] / T. V. Napadovskaya, Yu. A. Yakubovskaya // Lechebno-profilakticheskie meropriyatiya pri travmatizme i besplodii s.-h. zhivotnyh. – 1991. – S. 77–81.

21. Nahaenko A. V. Telyazioz krupnogo rogatogo skota (etiologiya, epizootologiya, patogenez, terapiya i profilaktika) [Telaziosis of cattle (etiology, epizootology, pathogenesis, therapy and prevention)]: avtoref. dis. ... kand. veterinar. nauk: 03.00.19 / Nahaenko Aleksandr Vladimirovich. – M., 1997. – 22 s.

22. Sivkov G. S. Vidovoj sostav vozbuditelej i rasprostranenie telyaziozov krupnogo rogatogo skota na yuge Tyumenskoj oblasti [Species composition of pathogens and distribution of telaziosis in cattle in the south of the Tyumen region] / G. S. Sivkov, V. N. Domackij, L. A. Glazunova // Problemy entomologii i arahnologii: sb. nauch. trudov VNIIVEA. – 2005. – Vyp. 47. – S. 114–118.

23. Glazunova L. A. Рazrabotka i usovershenstvovanie metodov terapii i profilaktiki telyazioza krupnogo rogatogo skota v Severnom Zaural’e [Development and improvement of methods for the treatment and prevention of bovine thelaziosis in the Northern Trans-Urals]: dis… d-ra veterinar. nauk / L. A. Glazunova. – Sankt-Peterburg, 2018. – 314 s.

24. Gusejnov N. G. Telyazioz krupnogo rogatogo skota [Telaziosis of cattle] / N. G. Gusejnov // Veterinariya. – 2010. – № 2. – S. 30–35.

25. Latypov D. G. Gel’mintozy krupnogo rogatogo skota v respublike Tatarstan [Helminthiases of cattle in the Republic of Tatarstan]: avtoref. dis. ... d-ra. veterinar. nauk (6 03.02.11) // Latypov Dalis Garipovich. – M., 2010. – 40 s.

26. –53. Vide supra.

27. Anisimova E. I. Spiruridy krupnogo rogatogo skota severo-vostoka Bol’shogo Kavkaza [Spirurids of cattle of the northeast of the Greater Caucasus]: avtoref. dis. ... kand. veterinar. nauk: 03.00.19 / Anisimova Elena Ivanovna. – M., 1984. – 20 s.

28. Ataev A. M. K telyaziozu krupnogo rogatogo skota v Dagestane [To telaziosis of cattle in Dagestan] / A. M. Ataev [i dr.] // Teoriya i praktika bor’by s parazitarnymi boleznyami: Mat. dokl. nauchn. konf. – 2002. – № 3. – S. 37–38.

29. Zubairova M. M. Zarazhyonnost’ krupnogo rogatogo skota telyaziyami v razreze vertikal’noj poyasnosti Dagestana [Infection of cattle with telasia in the context of the vertical zonality of Dagestan] / M. M. Zubairova, A. M. Ataev, N. T. Karsakov // Rossijskij parazitologicheskij zhurnal. – 2008. – № 3. – S. 1–4.

30. Zubairova M. M. Bioekologiya i epizootologiya telyazioza krupnogo rogatogo skota s uchetom vertikal’noj zonal’nosti regiona Severnogo Kavkaza [Bioecology and epizootology of telaziosis in cattle, taking into account the vertical zonality of the North Caucasus region] / M. M. Zubairova [i dr.] // Teoriya i praktika parazitarnyh boleznej zhivotnyh. – 2017. – № 18. – S. 185–187.

31. Kerimova S. Stepen’ zarazhyonnosti krupnogo rogatogo skota i bujvolov telyaziozom [The degree of infection of cattle and buffaloes with telaziosis] / S. Kerimova // Tr. Azerbajdzhanskogo SHI. – 1960. – T. 11. – S. 39–40.

32. Kolesnichenko M. L. Vozbuditeli telyazioza glaz u kopytnyh Azerbajdzhana [Causative agents of eye thelaziosis in ungulates of Azerbaijan] / M. L. Kolesnichenko, A. N. Gusejnov // Materialy nauchn. konf. VOG. – 1965. – Ch. 4. – S. 98-99.

33. Kejserovskaya M. A. Ekologo-geograficheskaya harakteristika i epizootologicheskie osobennosti rasprostraneniya vozbuditelej telyazioza krupnogo rogatogo skota, bujvolov i zebu v Azerbajdzhane [Ecological and geographical characteristics and epizootological features of the spread of pathogens of thelaziosis of cattle, buffaloes and zebu in Azerbaijan]: avtoref. diss. kand. biol. nauk: 03.00.19 / Kejserovskaya Marina Aleksandrovna. – Baku, 1969. – 18 s.

34. Aksenova P. V. Enukleaciya glaza u zubra. Klinicheskij sluchaj [Enucleation of the eye in bison. Clinical case] / P. V. Aksenova // Veterinarnaya patologiya. – 2021. – № 4 (78). – S. 57–61.

35. Vide supra.

36. Cibizova E. L. Metody regulyacii polovozrastnogo sostava zubrov v pitomnike Okskogo zapovednika [Methods of regulation of the sex and age composition of bison in the nursery of the Oksky Reserve] / E. L. Cibizova, P. V. Aksenova, A. M. Ermakov // Veterinarnaya patologiya. – 2014. – № 1 (47). – S. 49–54.

37. Cibizova E. L. Osobennosti proyavleniya reproduktivnoj funkcii u zubra evropejskogo (Bison bonasus) pri poluvol’nom soderzhanii v usloviyah pitomnikov [Peculiarities of manifestation of reproductive function in the European bison (Bison bonasus) with semi-free keeping in nurseries] / E. L. Cibizova, P. V. Aksenova, A. M. Ermakov // Veterinarnaya patologiya. – 2015. – № 1 (51). – S. 54–63.

38. Docev A. V. Issledovanie allelofonda i geneticheskoj struktury rossijskoj populyacii zubrov (Bison bonasus) kavkazsko-belovezhskoj linii [Study of the allele pool and genetic structure of the Russian population of bison (Bison bonasus) of the Caucasian-Belovezhskaya line] / A. V. Docev, Polina Vladimirovna Aksenova, Valeriya Vladimirovna Volkova, Veronika Ruslanovna Harzinova, Ol’ga Vasil’evna Kostyunina, Roman Astaketovich Mnacekanov, Nataliya Anatol’evna Zinov’eva // Ekologicheskaya genetika. – 2017. – T. 15. – № 2. – S. 4–10.

39. Aksenova P. V. Bolezni zubrov (Bison bonasus): vstrechaemost’ i epizooticheskie osobennosti zabolevanij zubrov bakterial’noj etiologii [Diseases of bison (Bison bonasus): occurrence and epizootic features of diseases of bison of bacterial etiology] / P. V. Aksenova // Veterinarnaya patologiya. – 2014. – № 2 (48). – S. 51–63.

40. Aksenova P. V. Opasnost’ mikoplazmoza dlya dikih populyacij zubra (Bison bonasus). Osobennosti epizootii i patogeneza [Danger of mycoplasmosis for wild populations of bison (Bison bonasus). Features of epizootics and pathogenesis] / P. V. Aksenova, A. M. Ermakov, L. P. Mironova, E. L. Cibizova // Rossijskij veterinarnyj zhurnal. Melkie domashnie i dikie zhivotnye. – 2014. – № 3. – S. 38–42.

41. Cibizova E. L. Dinamika i prichiny smertnosti zubrov (Bizon bonasus) v pitomnike Okskogo gosudarstvennogo prirodnogo biosfernogo zapovednika [Dynamics and causes of mortality of bison (Вizon bonasus) in the nursery of the Oksky State Natural Biosphere Reserve] / E. L. Cibizova, P. V. Aksenova, A. M. Ermakov // Veterinariya. – 2015. – № 6. – S. 13–17.

42. Aksenova P. V. Vstrechaemost’ i epizooticheskie osobennosti zabolevanij zubrov parazitarnoj etiologii [Occurrence and epizootic features of diseases of bison of parasitic etiology] / P. V. Aksenova // Veterinarnaya patologiya. – 2015. – № 1 (51). – S. 28–39.

43. Aksenova P. V. Bolezni zubrov (Bison bonasus). Vstrechaemost’ i epizooticheskie osobennosti zabolevanij zubrov virusnoj etiologii [Diseases of bison (Bison bonasus). Occurrence and epizootic features of bison diseases of viral etiology] / P. V. Aksenova, A. M. Ermakov // Veterinarnaya patologiya. – 2015. – № 3 (53). – S. 22–31.

44. Aksenova P. V. Risk razvitiya postanestezionnoj miopatii u zubrov [The risk of postanesthesia myopathy in bison] / P. V. Aksenova // Veterinarnaya patologiya. – 2018. – № 3 (65). – S. 52–60.

45. Aksenova P. V. Veterinariya zubrov. Prakticheskoe rukovodstvo [Veterinary medicine of bison. Practical guide] / M: Vsemirnyj fond dikoj prirody (WWF), 2020. – 224 c.

46. Aksenova P. V. Infekcionnyj keratokon”yunktivit u zubrov (Bison bonasus) [Infectious keratoconjunctivitis in bison (Bison bonasus)] / P. V. Aksenova // Veterinarnaya patologiya. – 2020. – № 2 (72). – S. 68–76.