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DETERMINATION OF MILK QUALITY BY BACTERIOLOGICAL AND EXPRESS METHODS

https://doi.org/10.25690/VETPAT.2020.72.2.010

Abstract

Rostov region and was examined in the Taganrog Interdistrict Veterinary Laboratory. In parallel with bacteriological studies, Petrifilm test plates were used to determine QMAFAnM. Milk samples were taken from each lobe of the udder during the morning milking. We examined milk in the first farm from 15 cows there were 60 samples. In the second farm, milk from 10 cows was examined there were 40 samples. In the first farm, in the study of milk from 15 cows by the bacteriological method, microorganisms were detected. Of the 60 milk samples, mesophilic aerobic and facultative anaerobic microorganisms (QMAFAnM), as well as somatic cells, were found in the 21st sample. Rapid test revealed a positive result in 10 samples of milk. Thus, the sensitivity of the rapid test is lower than bacteriological by 47.6 %. In the second farm, when examining milk from each udder share from 10 cows by the bacteriological method, mesophilic aerobic and facultative anaerobic microorganisms (QMAFAnM), as well as somatic cells, were found in 15 samples from 40 samples. Staphylococcus aureus was isolated in two milk samples. A positive result of the rapid test was obtained in 8 samples from 15 milk samples. The sensitivity of the rapid test was 53.33 %. Thus, the bacteriological method turned out to be more sensitive with the rapid test by 53.33 %. The results of the laboratory method and in parallel using the Petrifilm test plate coincided, but not in all samples, but only where the number of microorganisms was many times higher by the bacteriological method. The express method is specific, reduces the time of research.

About the Authors

A. V. Toropyno
(HPE) «Kuban State Agrarian University named after I. T. Trubilin»
Russian Federation


A. A. Shevchenko
(HPE) «Kuban State Agrarian University named after I. T. Trubilin»
Russian Federation


References

1. Емельяненко М. А. Ветеринарная микробиология / М. А. Емельяненко, Т. В. Дунаев, Д. Г. Кудлай и др. // - М.: Колос, 2001. - 304 с

2. Тарасов К. И. Проблема обеспечения качества и безопасности пищевых продуктов / К. И. Тарасов. Все о мясе. - Т. 2-3. - № 4. - С. 45-46

3. Андреев В. Б. Требования к качеству молока / В. Б. Андреев, Л. Д. Демидова, В. В. Ивановцев // Практик. - 2007. - № 4. - С. 20-23

4. Практикум по микробиологии / сост. А. Н. Нетрусов, М. А. Егорова, Л. М. Захарчук. - М.: Академия, 2005. - 608 с

5. Yang Y. Bovine leukemia virus infection in cattle of China: Association with reduced milk production and increased somatic cell score / Y. Yang, W. Fan, Y. J. Mao [et al.] // Dairy Sci. - 2016, May. - Vol. 99 (5). - P. 3688-97

6. Драчева Л. В. Приборы для контроля качества и безопасности пищевых продуктов / Л. В. Драчева // Масложировая промышленность. - 2007. - № 2. - С. 20-21

7. Caldow M. K. Short communication: Bovine-derived proteins activate STAT3 in human skeletal muscle in vitro / M. K. Caldow, M. R. Digby, D. Cameron-Smith // J. Dairy Sci. - 2015, May. - Vol. 98 (5). - P. 3016-9

8. Bhatia S. Serological evidence of bovine immunodeficiency virus infection in cattle and buffalo through use of recombinant capsid (P26) protein based immunoassay / S. Bhatia, A. K. Bhatia [et al.] // J. Immunol Immunopathol. - 2006. - Vol. 8. - № 2. - P. 128-129

9. Овод А. С. Ветеринарные мероприятия в молочном животноводстве / А. С. Овод, А. С. Ирский, Н. М. Сидоренко и др. // - Новочеркасск. 2003. - 224 c


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For citations:


Toropyno A.V., Shevchenko A.A. DETERMINATION OF MILK QUALITY BY BACTERIOLOGICAL AND EXPRESS METHODS. Russian Journal of Veterinary Pathology. 2020;(2):62-68. (In Russ.) https://doi.org/10.25690/VETPAT.2020.72.2.010

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