USING OF SELEN NANOPARTICLES AS CARRIER OF DRUGS AND ANTIGENS ON EXAMPLE OF ADJUVANT IN IMMUNIZATION ANIMAL AGAINST COLIBACILLOSIS
Abstract
The work was carried out construction of immunizing designs based on selenium colloids extracellular antigens and whole cell vaccine strain Escherichiacoli B-5 and studied the protective immunization of these drugs in laboratory animals. For immunization using nonlinear white mice and guinea pigs. Immunized intraperitoneally twice at an interval of 1 week. After the second immunization, animals were injected with LD 100 E.coli B-5 mortality rate in the Group accounts for 5 days. Previous immunization was carried out in 4 repetitions. As a result of carried out work, it was found that the selenium colloid, as an adjuvant to stimulate antibody production against the components of bacterial cells (the outer membrane lipoproteins, flagella, live E.coli cells) against hemolysin and its culture medium. Serum after immunization loses their growth properties. Immunized animals by selenium constructs show higher survival in comparison the control group vaccinated with the killed culture after infection with a lethal dose for intact animals.
About the Authors
Габалов
ФГБНУ «Саратовский научно-исследовательский ветеринарный институт»
Russian Federation
Russian Federation
Видягина
ФГБОУ ВПО «Саратовский государственный аграрный университет имени Н.И. Вавилова
Russian Federation
Russian Federation
Рюмина
ФГБНУ «Саратовский научно-исследовательский ветеринарный институт
Russian Federation
Russian Federation
Тарасенко
ФГБНУ «Саратовский научноисследовательский ветеринарный институт»
Russian Federation
Russian Federation
Сергей Староверов
ФГБНУ «Саратовский научно-исследовательский ветеринарный институт»;
Russian Federation
Russian Federation
Алексей Волков
ФГБНУ «Саратовский научно-исследовательский ветеринарный институт»;
Russian Federation
Russian Federation
Сергей Енгашев
ФГБОУ ВПО «Нижегородская ГСХА
Russian Federation
Russian Federation
Михаил Малинин
ФГБНУ «Саратовский научно-исследовательский ветеринарный институт»
Russian Federation
Russian Federation
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Review
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, , , , , , , USING OF SELEN NANOPARTICLES AS CARRIER OF DRUGS AND ANTIGENS ON EXAMPLE OF ADJUVANT IN IMMUNIZATION ANIMAL AGAINST COLIBACILLOSIS. Russian Journal of Veterinary Pathology. 2016;(3):31-38. (In Russ.)
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