STUDYING THE VIRULENT PROPERTIES OF THE FIELD ISOLATE OF BOVINE NODULAR DERMATITIS “GEGANUSH-2016”

Nodular cattle dermatitis is an economically significant transmissible infectious disease. The virus can cause a temporary decrease in milk production, temporary or permanent infertility in bulls, skin damage and death from secondary bacterial infections. Purebred animals are more susceptible to this infection, the most severe disease occurs in young animals, thin or lactating cows. Until 2015, bovine nodular dermatitis was never reported in Armenia. The first clinical cases of nodular dermatitis were noticed in the southern part of Armenia (near the border with Iran) at the end of 2015 among cattle that was kept on pasture. Samples taken from sick animals were positive for the nodular dermatitis virus by tested by real-time PCR. Examination in the Federal State Budgetary Institution “Federal Animal Health Center” (Vladimir, Russian Federation) confirmed the presence of the nodular virus dermatitis. For the first time, we estimated the pathogenicity of the eld isolate of the nodular dermatitis virus “Geganush-2016” isolated from cattle in the Syunik region of the Republic of Armenia in 2016. In an experiment with 6-month-old calves (n = 2), intradermal infection with a suspension of the field isolate of the nodular dermatitis virus “Geganush-2016” caused the formation of nodules on the skin at the sites of virus introduction. Nodules were benign, 2 weeks after their appearance, scabs formed, which then separated from the skin, leaving small scars. After euthanasia, blood samples, exudates, areas of skin and samples of internal organs (spleen, kidneys, liver, mucous membranes, affected areas of the pharyngeal, submandibular and mesenteric lymph nodes, tonsils, lungs, heart) were taken to study. The genome of the virus was found in the mucous membranes, the affected areas of the pharyngeal, submandibular and mesenteric lymph nodes, tonsils, skin, tubercles, muscles and exudates

virus, nodular dermatitis, nodular exanthema, infectious disease, skin nodules, biological properties, isolate, cattle, pathological material

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