No 1 (2014)
ВНУТРЕННИЕ БОЛЕЗНИ
5-8 124
Abstract
The paper reports results of comparative analysis of Geptral 24.6 mg/kg, Lipoton 5 mg/kg, and Gamavit 0.5 mg/ kg in treatment of chronic hepatopathy in dogs. The goal was to conduct comparative study of hepatoprotective properties of the preparations. The experiment enrolled 30 dogs with chronic liver injury manifestations proved by biochemical blood serum analysis. Pharmacological activity of the test drugs was evaluated through clinical examination of the animals, and evaluation of the blood serum biochemical properties representative for liver functions. Test preparations were introduced intramuscularly once a day for one week. The treatment resulted in apparent improvement of liver functions proved by changes in the blood biochemical analysis. Total blood protein raised by 28.6% in Geptral, by 30.7% in Lipoton, and by 29% in Gamavit therapy groups against pre-treatment level. Bilirubin content before treatment was 6.3 ± 0.18 mmol/l as a result of either excessive production or ineffective conjugation in the liver. Following the treatment the value decreased by 20.6% (Lipoton), 19% (Geptral), and 15.8% (Gamavit). This may indicate restoration of pigment metabolism functions in liver. Blood ALT and AAT levels were elevated (84.2 IU/l and 72.8 IU/l respectively) before the treatment. Geptral was the most effective in decreasing content of the enzymes (AAT decrease by 1.7 times, ALT decrease by 1.7 times as compared with pre-treatment values). ALT and AST intracellular enzymes occur in small amount in the blood of healthy animals, and their elevation indicates cell lysis. The study proves efficacy of Lipoton and Gamavit preparations, and validates their use in hepatopathy therapy in dogs.
Lyudmila Chizhova,
Antonina Kuzminichna Mihaylenko,
Aubekir Umarovich Ediev,
Cholpan Bilyalovna Chotchaeva
8-12 60
Abstract
The study was based upon analysis of age-specific variation in energy exchange metabolites content in the peripheral blood in sheep raised in different environmental and climatic zones featuring unequal iodine availability. The following values were used to estimate correlation between intensity of energy balance and functional activity of thyroid gland: total lipids, cholesterol, and glucose in the blood of sheep raised in lowlands (plain) and in mountains with different iodine availability, and examined at different stages of growing and development of the animals. It was found that intensity of energy balance is both age and environment specific. In both cases deficiency of energy products play significant role: it relates either to the organism’s demand during intensive growth or to insufficient supply of thyroid hormones in case of limited functional activity of thyroid. The results indicate to a certain degree level of health in the animals subjected to iodine deficiency, and may be used to develop programs, recommendations or measures on elimination of iodine deficiency.
ИНФЕКЦИОННЫЕ БОЛЕЗНИ
13-21 81
Abstract
Objective: to perform complex examination of cows during calving and prophylactic follow-up period and derived infected calves. To assess selectivity and diagnostic value of indirect (IIAT) and direct (DIAT) immunofluorescence antibody tests in mixed infections manifestation. To establish localization, structural composition, ways of microbe extraction, and infection mechanisms. Biological material used in life-time diagnosis was: calves’ bronchioloalveolar mucus obtained by alveolar lavage; blood serum, mucus and feces from straight intestine. Blood serum, udder fluid, and urogenital tract secreta were tested in cows. We developed and applied methods of serum diagnosis through direct and indirect immunofluorescence (DIAT and IIAT) for life-time and postmortal detection of antigens and antibodies. Growth media developed in cooperation with Omsk Scientific and Research Institute of Natural and Focal Infections of Rospotrebnadzor were used in bacteriological tests. Complex tests for antibodies and antigens using DIAT and IIAT allowed detection of 10 to 89% of animals (cows and calves) carrying IBR-PV, Salmonella, Chlamydia, Leptospira, and Listeria in various combinations in addition to responsive animals. Associations of causative agents of Chlamydia infection, Q-fever, mycoplasmosis, IBR-PV, leptospirosis, and listeriosis were found in urogenital tract of 20 to 50% of cows after calving and in 10 to 50% of udder fluid samples. Conclusion: 1. DIAT and IIAT are highly sensitive diagnosis tests providing for rapid complex life-time examination of biological materials for presence of antibodies and antigens in the blood, intestine, respiratory, and genital systems and udder in animals; moreover, these diagnosis tests were validated for detection of mixed infection manifestation in cows during calving and prophylactic follow-up period and in delivered calves as well. 2. Complex examination of infected calves in two age groups allowed detection of 10 to 40% and 10 to 89% respectively animals carrying and extracting with feces, bronchioloalveolar and cervicovaginal mucus, milk, and beestings Streptococcus, Staphylococcus, Diplococcus, enterotoxigenic E. Coli, Chlamydia, IBR virus, Salmonella, Leptospira, Listeria, Coxiella, and mycoplasms in addition to responsive animals. 3. Calves are generally infected with Chlamydia, Leptrospira, Listeria, Salmonella, Coxiella, IBR-PV, and mycoplasms transplacentally, while passing through parturient canals during calving, or with milk and beestings.
Vyacheslav N. Vasilenko,
Sergey N. Kartashov,
Lyudmila P. Mironova,
Alexey M. Ermakov,
Anna A. Mironova
22-27 51
Abstract
African Swine Fever Virus (ASFV) penetrated Rostov Oblast from southern regions of Russian in 2009, the fact is supported by official letters of the Federal Service for Veterinary and Phytosanitary Surveillance. During the reported period, 81 cases of ASF were recorded in 26 districts and 3 towns of Rostov Oblast, including 66 cases among pigs in farms, and 15 cases in wild boars. Introduction in 2010 and strict compliance with the system of total control yielded desired results: no secondary niduses of infection have been registered since late August that year. Subsequent cases of ASF in the Oblast were mostly attributed to the virus introduction from neighboring infected regions, with wild boars in some cases. Challenge to protect pig livestock against ASF and budget against losses will stay urgent for a long time on. African Swine Fever in Russia has long ago stepped beyond any individual region. The procedure developed in practice in Rostov Oblast makes it possible to control ASFV spreading across the region, is helpless against virus introduction from beyond the Oblast. To ensure protection against virus external intervention it is necessary to tighten supervision over import and interregional transport of live pigs and pig farming products without heat treatment. This is attainable in close cooperation and coordination between Federal and regional veterinary services, and internal affairs authorities only.
27-31 87
Abstract
Tolerance to Leptospira in piglets may be induced on purpose by introducing avirulent Leptospira of certain serogroups to the new-born animals before first sucking of beestings. Experiments were executed on piglets. Five pregnant sows and one sow with 14 days old piglets were selected for the experiment at a leptospirosis-free farm. The sows were quarantined and examined for Leptospira and antibodies to Leptospira. The results were negative. Right after farrowing and before first sucking of beestings the piglets were isolated and intraperitoneally inoculated with avirulent Leptospira antigen of Pomona serogroup. The piglets were raised on artificial feeding. Twenty five days after the first inoculation each piglet was intraperitoneally inoculated with 2 ml of virulent Pomona serogroup culture. Challenged piglets were observed for 20 days more. It was found that introduction of 2 ml of live avirulent Leptospira culture (gr. 3) resulted in tolerance in up to 33% piglets while the same dose of dead Leptospira generated tolerance in 25% of piglets. Introduction of avirulent antigen on the 14th day after birth resulted in tolerance in 14.3% of piglets only. High ratio of Leptospira carriage (18.2%) among areactive (tolerant) animals is a substantially hazardous source of infection. It is necessary to screen for Leptospira carriage all pigs transferred to other farms for breeding purposes, including animals seronegative to leptospirosis. This requirement must be mandatory for all farms with history of leptospirosis.
32-39 66
Abstract
Sometimes fodders of plant or animal origin intended for poultry production do not meet biological safety requirements and are impaired by fungi-producing mycotoxins resulting in huge economic loss. Yet there have been developed and tested feed additives based on the natural minerals and biologically active agents (vitamins, organic acids) - ekobentokorm and adsorptive vitamin and mineral feed additive (AVMFA), which are capable of neutralization of mycotoxins, enrich fodder with highly biologically available minerals and vitamins, thus enhancing digestion, diminishing toxic load, improving fodder safety and productivity, and upgrading fodder composition and ecological sustainability. Morphological, biochemical, and immune status was found improved in the blood of egg-laying hens when fed with compound fodder supplemented with adsorbing additives which enhanced oxidation and reduction processes and protein assimilation in the bird’s organism. These changes resulted in improvement of morphological and biochemical characteristics of eggs thus promoting their biological and nutrition value. To stimulate egg-laying poultry production adsorbing additives ekobentokorm and adsorptive vitamin and mineral feed additive (AVMFA) were introduced in amount of 3.0% of the ration mass. It was found that the most advantageous additive to the compound fodder was adsorptive vitamin and mineral feed additive (AVMFA).
39-45 60
Abstract
The study was aimed at determination of morphometric characteristics of mink’s stomach: mass, volume, length, stomach mass to body and gastrointestinal tract mass ratios, and age-dependent coefficient of growth intensity subject to different motion activity levels. We used micro- and macroscopic morphometric methods. During eight months of experiment, average mass of stomach in males in the test group increased by 49.2 times (control - by 48.5 times); in females in the test group the growth was 57.7 times (control - 53.3 times). During the same period stomach volume in males increased by 238.5 times in test group (control - by 238.0 times); in females the growth was 115.5 times in both groups. Stomach length in males in both groups increased by 9.8 times, in females - by 8.1 times in test and by 8.8 times in control group. Postnatal age-related morphological ontogenical changes of stomach in minks develop unevenly and asynchronously. There are four periods with different pattern and intensity of stomach growth in minks: one month from birth - the most intensive growth; two months to four -gradual slow-down of the stomach growth; four months to eight months - stabilization of characteristics. Sexual dimorphism was observed in the stomach growth pattern: morphometric characteristics in males were higher than in females. Additional daily motion activity in minks positively influenced stomach growth resulting in steady tendency of higher morphometric characteristics in the test group over control.
ОБЩАЯ ПАТОЛОГИЯ
46-49 159
Abstract
We have performed a study at the veterinary surgery subdepartement of the Moscow federal state budgetary institution of higher and professional education “MGAVMiB after K. I. Skryabin” under general direction of doctor of veterinary science, professor, director of the subdepartement Filippov Yu. I., aimed at corroboration of uniqueness and effectiveness of GDV bioelectrophotography as a method of monitoring and check of individual organs or organ systems during therapeutic interventions. Dogs of giant breeds (Caucasian Shepherd, Dogo Argentino, and Dogue de Bordeaux) with a range of diagnosed acute knee joint pathologies (acute arthritis of knee joint, acute synovitis of knee joint, and sprain of anterior cruciate ligament of knee) were used in the test. The diagnoses were clear and confirmed with standard methods, including laboratory ones. Examination was carried out with gas-discharge visualization (GDV) apparatus equipped with accessory ACU scanner for BAP imaging using GDV Capture software. GDV Scientific Laboratory software was used for parametric evaluation of GDV-grams. Each point was photographed without a filter in automatic mode for a certain time (with 10 sec. interval). To maintain maximum validity, the experiment was held on each animal several times in different days. Initially the image was taken on diagnosis during acute period before treatment (either medicamentous or surgical), then on day 3 and day 7 since therapy onset (3 times in total). A number of interrelated criteria were evaluated in every GDV-gram of a BAP: entropy, area, and form-factor. A conclusion may be drawn based on the complex of the abovementioned parameters: first, numerical ratios were similar in all dogs; second, image area increased with therapy progress, while entropy and form-factor values decreased. It implied inflammatory process dissipated (if an inflammatory process was present in the animal, BAP glowing area decreased too revealing energy deficiency). Some experts believe that area below 21 thousand pixels implied inflammation (initial glowing area in the test animals varied between 21,000 to 23,000 pixels, clearly indicating beginning of degenerative changes). The study clearly showed that gas-discharge visualization provides for highly reliable method of real-time health monitoring. It is also meaningful diagnostic technique applicable for monitoring and treatment prescription.
49-54 65
Abstract
The study deals with the main reasons for discarding bison Bison bonasus and methods of age and gender composition control in the bison population in the nursery of the Oksky Nature Reserve. The authors studied discarding process and age and gender structure of the animals removed from the bison nursery. It was found out that the main reason for breeder bison discarding was complete or partial loss of reproductive capacity due to old age or trauma. This group covered 70.8% of the total number of discarded animals. In total 24 animals were culled by shooting during overall period of the nursery operation, which is 15.2% of overall bison population loss in the nursery. Current policy of the nursery provides for rather more rational and humane approach to control age and gender structure of bison population. First of all, this involves optimal composition of the groups released into the wild. This implies introduction into existing or new group an old female leader along with young animals for replacement. This leadership ensures the young animals readily restore after relocation stress, adapt to new environment; minimizes accidental losses and traumas in the herd, and eliminates hierarchical struggle and fights in the first several years. Later on, after the older animal dies, a new female naturally takes on leadership accepting the same pattern of protective behavior adopted from the predecessor. Males out of reproductive age are unnecessary in wild populations; therefore they are taken to display enclosures. This approach provides for complete abandon of culling in the nursery.
Mariya A. Bodryakova,
Leonid N. Fetisov,
Alexander A. Zubenko,
Anatoliy N. Bodryakov,
Alexander V. Kovalenko,
Lyubov’ A. Lyashchenko,
Elena V. Zhila
55-60 48
Abstract
Development of resistance to chemotherapeutic agents in microbiota is one of the major challenges of infectious diseases therapy. Veterinary specialists need resources to substitute inefficient agents with drugs to which resistance development is impossible or takes much time. Hence it is necessary to synthesize and screen new agents with high antimicrobial activity, low toxicity, inexpensive, and easy-to-use. Antibacterial activity of new agents from fatty acid amides series was studied on E. coli 078 (field strain) and St.aureus P-209, leading to selection of prospective compounds with high specific activity and no acute toxic effect. Fatty acid amides have potential to be used as active ingredients for new veterinary preparations.
ФИЗИОЛОГИЯ
60-66 49
Abstract
Adaptive capacity has been studied in imported sows of the following Dutch breeds under the conditions of Kuban industrial farming: Landrace (L), Duroc (D), and crosses Landrace x Yorkshire (L x Y). Experiments have shown that hematological factors in the imported animals varied within normal range. Nevertheless, a slightly elevated white cell count in imported animals (L, L x Y, and D) by 0.46, 0.41 and 0.21x109/l respectively as compared to local pigs (KB), indicates some “stress” and disturbance in the immunity function. Further, slight elevation of erythrocyte count in imported breeds 6.87х1012/l, 6.75х1012/l and 6.82х1012/l against 6.22х1012/l also indicates some internal processes of immune system stabilization. Total protein content in the blood serum was rather high and uniform in all breeds. Elevated . - globulin faction indirectly shows stress in the imported animals, which may result from abrupt change in feeding, housing and environmental conditions. Besides, our study has established that natural resistance is higher in indigenous KB breed. Thus, phagocytic activity in KB breed was higher by average 2.8%, phagocytic count by 5.6%, phagocytic capacity by 2.3%, and by phagocytic index by 6.8%. The value is more pronounced in the area- specific animals of the KB breed. At the same time, the index may be substantially leveled if stable indoors conditions preserved (23°C). We consider R. Benezra’s approach which takes into account the breathing rate, the most unbiased method of evaluation of adaptive capacity in animals to certain environmental conditions. Therefore, imported Dutch breed animals have high adaptive capacity and satisfactory pass acclimatization in the Kuban region under industrial farming conditions.
66-71 60
Abstract
Efficiency and competitiveness of present-day sheep breeding depend on the fuller utilization of the sheep meat production. Therefore we work on improvement and enhancement of Merino sheep meat-making properties by using breeding stock from global gene pool. Nevertheless, raising productivity in sheep requires knowledge of physiological and biochemical mechanisms underlying development of an animal and formation of their productivity. On this assumption we have studied blood morphological composition, protective potential, and revealed differences between young sheep of different breeds obtained from Soviet Merino ewes mated with linear rams of Australian Merino, Australian Meat Merino, and half-blooded Australian Meat Merino. Hematological factors (erythrocytes and leucocytes counts, hemoglobin level) and resistance characteristics (bactericide and lysozimic activity of the blood serum) were determined by generally accepted methods of analysis in young ewes of 4 months old, before feeding. Analysis of blood morphology data from younglings with different genotypes revealed that cross-bred young ewes of I, II and III groups had higher erythrocyte counts (4.3 - 12.4%) and maximum hemoglobin level (7.0 - 11.6) in blood as compared to purebred peers of the same age. Evaluation of protective potential in different sheep breeds showed that cross-bred young ewes (I, II and III groups) had advantage over purebred animals in terms of natural resistance characteristics. Further, it was detected that humoral immunity in cross-bred younglings (I, II and III groups) was 0.73 - 2.2 and 0.064 - 1.7% higher than in Soviet Merino peers of the same age. Study of hematological profile and immune reactivity indicate advanced development of natural protective factors and elevated redox processes in the cross-bred animals resulting in productivity. Among cross-bred younglings with different genotypes the best morphological blood composition and elevated natural protection potential were found in progeny from Australian Meat Merino.
71-75 83
Abstract
Rupture of anterior cruciate ligament is a frequent pathology in large and giant dog breeds. Objective: To study a method of tuberosity advancement (TTA) on the tibia in treatment of the anterior cruciate ligament rupture in dogs. The study comprises results of the tibia tuberosity advancement in surgical treatment of anterior cruciate ligament rupture in 55 dogs of different breeds. The dogs were 3.5 to 6.5 years old. Body weight of the animals varied 49.9 ± 6.5 kg. Therapy results are represented in terms of the joint stability and instability analysis, local temperature and edema, occurrence of para-articular structures, and painful movement; complication were found in 10% cases. At the same time, no implant rejection was observed during post-surgery period. Conclusion. Strict adherence to the method of tuberosity advancement on the tibia in therapy of anterior cruciate ligament which involves elimination of crescentic cartilage ruptures, and introduction of a spacer into the diastasis between the tuberosity and tibia fixed with a retainer plate, was found beneficial. This method is widely available, effective, and may be recommended for therapy of anterior cruciate ligament in large or giant dog breeds.
M. Yu. Novikov,
L. S. Litvinova,
V. V. Shupletsova,
A. G. Goncharov,
N. N. Shusharina,
E. A. Bogdanov
76-80 73
Abstract
Topicality. Researches in the field of surmounting resistance mechanisms require considerable financial investments and fall behind dynamics of resistance formation in microorganisms. Antiseptic properties of silver make it possible to use it as anti-infection agent. Objective: to estimate effectiveness of silver-containing dressing materials (from different manufacturers) used in veterinary to treat infected wounds. Material and methods. The study encompassed 21 animals (dogs) with wounds with different etiology (traumatic, pressure sores from fixing splints, scalping-type) on distal parts of limbs. The wounds were contaminated with microflora without apparent abscess. Normal skin microbiota provided the causative agents: S. epidermidis and S. Pseudointermedius. Clinical presentations, type, size and location of wounds, transitory complications, treatment progress, as well as breed, sexual, and age-specific characteristics of animals were recorded in the treatment protocols. Three specimens of dressing material were tested; all were silver-containing antimicrobial dressings from different producers (Silvercel, UK; Atrauman AG, Germany; and dressing with nanostructural silver coating, FSImmanuil Kant Baltic Federal University, Russia) applied to wound treated with anti-infection agent (chlorhexidine). Findings. Relatively similar curative effect of silver-containing dressings by different manufacturers leads to conclusion that Russian dressing material is the most economically advantageous in terms of optimum cost/quality ratio.
I. I. Mikhailova,
T. R. Leshchenko,
V. I. Iltchenko,
O. N. Mikhailova,
Ja. A. Bratskih,
A. V. Vasiliev,
I. V. Ulanov,
E. Ju. Finageev,
V. D. Iltchenko
80-87 86
Abstract
One of prospective lines of livestock farming progress and success relies on intensification of cattle-breeding, but this efficient process is somewhat impeded by high occurrence and rate of non-contagious diseases among livestock, and specifically widespread and mass are ulcerous lesions on digits in cattle. A number of approaches, methods and techniques of treatment of this pathology in cows have been developed by today already. Nevertheless, most of the methods and techniques are time and labor consuming or lead to milk quality deterioration down to discarding, especially if treatment is conducted in milk cows, thus resulting in extra economic loss to livestock farmers. Taking all these shortcomings into consideration we have developed and provided a different procedure of cow therapy, which involves surgical treatment of pathologic area on the tows, sprinkling the ulcer with Edis powder, and covering it with protective bulky dressing. Further, the ulcerous area is treated through the dressing with laser beam from Reks apparatus produced by Petrolazer Group, without additional fixation of animal’s leg. This treatment method and procedure implies parenteral administration of antibiotics and other medicines, thus eliminating their entering into milk; therefore the milk from treated animals may be consumed by human unrestrictedly. Moreover, the number of redressing on cow legs is reduced, treatment takes less time, and stress to animals which may lead to milk yield is decreased; at the same time, laser therapy requires no additional labor time or service personnel involvement. All these advantages in general decrease cost of animal treatment and increases additional cash flow from milk sale; therefore, proposed therapy method is more cost-effective, economically advantageous, and most expedient for practical application in veterinary.
87-92 98
Abstract
Laparoscopic surgeries in animals require pneumoperitoneum which results in changes in the alveolar ventilation and hemodynamics due to excessive pressure in the peritoneal cavity. To prevent post-laparoscopy complications it is necessary to study character of hematological changes resulting from carbon dioxide pneumoperitoneum. Objective of the study was to determine informative value of the study and details of blood gases change during laparoscopic surgery. Ten apparently healthy dogs were enrolled into the study. The blood was taken from cephalic vein of the lower arm at the beginning, at the 20th and at the 40th minute of elective ovariectomies. It was found, that gas analyzer is effective for monitoring of animal’s homeostasis during laparoscopy. Further, it was found, that carbon dioxide pneumoperitoneum at 7 mm Hg resulted in changes in cell count, electrolytic and gas composition of the vein blood (pCO2, HCO3, AnGap, tco2, Na+, k+, Cl-) which varied within physiological norm, excluding (рСО2), which increased by 12.5% (from 48 ± 1.44 mmol/L at the beginning of the surgery to 54 ± 1.44 mmol/L during the surgery) without any serious adverse effect and may be balanced with superoxygenation in spontaneous breathing.
92-97 89
Abstract
Our study was focused on gas-discharge visualization as a mean of diagnostic of patella displacement in small dogs. We intended to show that bioelectrophotography provided very important method of pathology process and health state evaluation in animals. Our research was based on the readings of GDV-chamber, an instrument developed by Russian scientists for visualization and recording of gas-discharge glowing effects. This high-tech precision equipment allows real-time monitoring of biofields in test animals. Diagnosis of pathologic processes in the patella is impeded by unclear and implicit clinical manifestations (absence of pain, claudication). Repeated displacement of patella from its sliding path causes arthrosis of the knee-joint. Our clinical trials carried out with GDV apparatus were aimed at diagnosis of patella pathologic processes (including displacements) in small dogs. All animals were divided in 2 groups: healthy and with patella displacement. Examination was performed in the GDV-chamber and images were recorded in static mode using GDV Capture software. Data was processed with GDV Scientific Laboratory software. Analysis of observation results showed bursts beyond general contour, apparent changes in the distribution of glowing, and disrupted glowing corona of the leg in virtually all dogs with patella pathology. Experimental data was analyzed based on such characteristics of GDV-grams as entropy, area, average intensity, and length of isolines (either with filter eliminating effect of perspiration and gassing on skin or without). Resulting statistical calculations and analysis reliably validated significant difference in GDV-gram characteristics in sick and healthy dogs. Application of gas-discharge visualization (filter mode seems more accurate and correct) along with other diagnostic means provide for sufficient
98-103 106
Abstract
Epizootic process of bovine leucosis has been keeping at invariably high rate for many years. Measures taken against the disease address neither its source nor transmission of the causative agent. Both factors provide for stability of epizootic situation despite of removal of infected animals from herd with the initial signs of hematological stage of the disease. Therefore, existing antiepizootic measures are inadequate to oppose epizootic process of bovine leucosis. Based on own many years experience, review of literature sources, and data on bovine leucosis epidemic process in different regions of the Russian Federation, the author concludes that epizootic process of bovine leucosis consists of a regular transfer of virus from infected cows to the offspring. Initially only a small amount of blood corpuscles are infected with virus. At this stage the disease is undetectable even by means of immunodiffusion test (IDT), and therefore called asymptomatic. As the causative agent expands aggression against the blood corpuscles the disease enters the next stage when it can be detected with IDT. This stage is called serological. Further virus aggression leads to the hematological stage. It progresses with increasing number of infected blood corpuscles and affection of hemopoietic organs, resulting in development of tumors and death. The author suggests IDT-screening of all young heifers at the age of three, six, nine and twelve months with separation of positive test animals and the cow into isolated feeding groups, thus ensuring both improvement of leucosis situation at a farm in 3 - 5 years and devastation of the very causative agent of the infection.
104-108 73
Abstract
Objectives. To examine broiler and meat-and-egg breed chickens for cryptosporidia occurrence in several poultry- farms of the Ukraine. To calculate extensiveness of infestation. Materials and methods. The study was conducted at the premises of Odessa Experimental Station of the National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine” (NSC “IECVM”). Cryptosporidia screening covered 50 cage-kept broilers of 27 days old delivered from OJSC AGRODIBROVKA in Kiev Oblast; and 70 meat-and-egg breed chickens (Adler Silver breed) of 21 days old at NIVA ALLC in Odessa Oblast; and 65 floor-kept meat-and-egg chicken (Adler Silver breed) of 30 days old at FE BILYACHAT in Odessa Oblast. Standard screening practices were used. Fecal samples were taken individually from cloaca and direct smears prepared following standard procedure. Seven chickens were dissected to confirm diagnosis: 3 broilers from OJSC AGRODIBROVKA, and 2 chickens from NIVA ALLC and FE BILYACHAT each. Dried smears were stabilized and stained using Ziehl- Neelsen method and studied under 4x10 immersion microscope. Findings of the study. All three examined poultry farms in Odessa and Kiev Oblasts of the Ukraine were found infested with cryptosporidiosis. The diagnosis was confirmed in laboratory and postmortem dissection. Cryptosporidium oocysts found in the fecal smears were seen as rounded red-stained formations of 5.7 x 4.6 .m in average size. Based on the registered oocyst size it was concluded that the parasite was C. baileyi. Oocysts were found in 4 samples of intestine lavage and smears from small, large bowels and cloaca of the 7 dissected chickens. Extensiveness of chicken infestation with cryptosporidia varied from 18% to 31.4%. Maximum extensiveness was found among floor-kept meat-and-egg Adler Silver breed chicken of 21 days old from NIVA ALLC in Odessa Oblast; the least extensiveness of manifestation was among cage-kept broiler chicken of 27 days old from OOO AGRODIBROVKA in Kiev Oblast. Conclusions. Cryptosporidiosis was found among both cage and floor kept broiler and meat-and-egg breed chicken from certain poultry farms in Odessa and Kiev Oblasts of the Ukraine. Extensiveness of infestation was 18.0% among broilers and 27.6% to 31.4% among meat-and-egg chicken. Diagnosis of cryptosporidiosis was confirmed by chicken postmortem examination, microscopic examination of Ziehl-Neelsen stained smears, and discovery of C. baileyi cryptosporidia in the gastrointestinal tract.
ISSN 2949-4826 (Online)