In the production of vaccines, there is a decrease in the intensity of bacterial mass accumulation often. The reason for this problem is a decrease in the quality of raw materials for nutrient media. In the production of vaccines against listeriosis in farm animals, we have proposed biologically active substances based on embryonic quail tissues, which have potential stimulating properties with respect to listeria. The aim of the study was a comparative evaluation of the growth-stimulating effect of FGEP and CPMP on Listeria monocytogenes (vaccine strain «AUF»). During of the study the optimal stimulating dose of the studied substances was established. It is 1 % to the volume of Hottinger agar. The best stimulating effect to L. monocytogenes has CPMP, the addition of which to the Hottinger agar increases the number of colonies by a factor of 1,7 compared with the medium without additives (control). There is no negative effect of substances at a dose of 1 % on the morphological, cultural, tinctorial, biochemical, hemolytic properties and mobility of L. monocytogenes. The data obtained open up prospects for using the investigated growth stimulants in the vaccine production cycle against listeriosis in farm animals.

In connection with the uncertain taxonomic position of the causative agents of listeriosis and pigs erysipelas and the complexity of their identification, comparative studies of microorganisms on a number of chemical properties, metabolism indices in vitro cultivation, nutritional needs were carried out. The obtained data confirmed the similar of pathogens on the amino acid composition and the content of cytochromes. However, these microorganisms differ significantly in the characteristics of their metabolism. The metabolism of Erysipelotrix rhusiopathiae is characterized by a pronounced need for nitrogenous components of the medium, primarily in amino acids; The splitting protein, accumulation of amine nitrogen and ammonia are more pronounced. Arginine is split on the arginine dihydrolase pathway, which plays the role of an energy factor for microorganisms. Consumption of arginine in Erysipelotrix rhusiopathiae with formation of ornithine and ammonia can be considered as a diagnostic sign for this microorganism. Listeria has a high demand for carbohydrates, which shows an increase in growth activity in glucose-containing media, the accumulation of glycolysis products (a decrease in pH, the formation of organic acids, including keto acids), and the consumption of reducing substances. The formation of acetylmethylcarbinol is characteristic only of listeria, which is their diagnostic sign.

The protection of animals against classic infectious diseases through vaccination should be supplemented with knowledge of factor infectious diseases and integrate them into a single paradigm for the prevention of diseases of productive animals. It is advisable in this regard to significantly change the training program for veterinarians.

At the end of the 19th century rabies was widespread in the Belgorod region. The reason for the spread were stray dogs and wolves. For the first time, rabies in animals in the veterinary reporting was point in the 1980s. As a rule, only losses of farm animals were recorded. In reports there are no cases of rabies in dogs and cats, therefore the true size of the spread of rabies is unknown. Such statistics were observed in all reports of Zemstvo›s veterinarians (Zemstvo is the elective district council in prerevolutionary Russia). In all cases, when people were bitten by rabid dogs, they were sent to Moscow and Kharkov for treatment at the expense of the Zemstvo. In the XIX century in the villages, this disease was widespread, and the population often resorted to the help of «folk healers». At the end of the XIX century in Russia there was no special legislation on infectious diseases of domestic animals, except for rinderpest. Zemsky regulations on the prevention and cessation of infectious diseases determined the statute of the medical police. The first rules on measures to prevent and stop rabies in the province were worked out by the first congress of veterinarians of the Kursk province in 1894. According to these rules, animals, sick with rabies, were immediately killed. Was years when the rabies was developed into epizootics. All this spoke about the need to take urgent measures to combat this disease.

The purpose of this study is to determine the hemolytic toxic effect of helminthological dissection. Collection of invasive material was carried out in 2015-2016. In the hunting economy and reserves of the Central region of Russia. The material for the study was the owners of zoonoses, wich shot by hunters and caught in traps on one-time licenses. The age of the animals was determined by the teeth condition. Nine types of helminthozoonoses were detected, of which the causative agents of trichinoses and alveococcosis are especially dangerous. Diseases are noting in the three regions - Ryazan, Vladimir and Moscow. The final and the hosts of helminths are 7 species of predators and one omnivore. The fox plays the leading role in the spread of helminthozoonoses. The greatest lesions are observed in 3-5 years old and older animals.

This article is devoted to the case of babesiosis in a dog with a new for Russia etiological agent B. gibsoni. Now there are three forms of babesiosis (pyroplasmosis) in dogs. The first etiologic form of babesiosis is widespread in Europe and Russia. It is caused by B. canis and B. vogeli. The second form is found mainly in Africa, its causative agent is B. rossi. The third form is considered endemic for Asia, Africa and North America, its causative agent is B. gibsoni. Its presence in Europe and Russia is questionable. We found the presence of Babesia gibsoni into the parasitofauna of the Rostov region. The disease with babesiosis was atypical. There was an unstable appetite, periodic, weak and pallor of the mucous membranes, hyperchromic anemia, neutrophilic leukocytosis, thrombocytopenia, and slight increase in hepatic transferases and alkaline phosphatase. It was determined that in complex cases of recurrent anemia of unknown etiology, it is necessary to exclude the causative agent of babesiosis by the PCR method.

The purpose of this work was to study the possibility of using a preparation based on succinic acid and an organic phosphorus compound for the correction of hepatoxic syndrome in cows with subclinical ketosis of (Department of therapy and clinical diagnostics with roentgenology of the Kazan State Medical Academy). Criteria for the effectiveness of the drug were the blood values that were studied within 10 days. The total protein, albumin, total bilirubin, activity of aspartate and alanine transaminase, alkaline phosphatase and gamma-glutamine transferase were determined in the serum. In a clinical study, cows with subclinical ketosis also established a violation of the cud, dystonia of proventriculus, marked liver increasing and tenderness. Biochemical status was characterized by hyperbilirubinemia, disproteinemia, disfermentemia. Application of the drug three times in doses of 10-15 ml per animal is increasing the percentage of aminotransferase by 10-35 %, gammaglutamyltransferase by 35 %, serum total bilirubin content by 50-60 %.

The article the problems of digestion disorders in rumen and the chronology of the development of metabolic acidosis are shown. When producing significant amounts of lactic acid from starch concentrates, as well as feeding in large quantities of acidic foods, it is natural that the rumen becomes acidified. Acidic medium is unfavorable for reproduction of rumen microflora, utilizing lactic acid. Absorbed into the blood, it enters the liver. When the pH in the rumen is reduced to 5.5, a more severe form of acid-base disorder (metabolic acidosis) develops. Metabolic acidosis is the acidification of all body fluids. The danger of metabolic acidosis development lies in the fact that it causes a lot of various shifts in the activity of the organs and systems of the body and is accompanied by interdependent disturbances in the function of blood, blood circulation, respiration, liver, kidneys. When the organism is acidified, vital microelements are poorly absorbed, and some of them, such as Ca, Na, K, Mg, Fe, are intensively removed. Known means and new approaches to the prevention and treatment of these diseases are presented. The possibility of using succinic acid and its salts for the relief of metabolic acidosis is discussed.It has been established that enteric single application of succinic acid, in the amount of 15-25 g, on cows with an average body weight of 550-600 kg, provides a marked improvement in pathobiochemical processes in metabolic acidosis. A similar effect is achieved with parenteral administration of sodium succinate or ammonium succinate

The influence of iodine-containing preparations on the quality of turkey meat has been studied. The authors assessed the quality of meat, the level of amino acids and vitamins. The use of iodine-containing drugs had an uneven effect on the meat productivity of turkeys. The weight of the half-dead carcass was also higher in the experimental groups (by 2.19, 2.53 and 2.35 kg). The slaughter yield of the carcasses of the test groups was 0.71-4.18 % higher than in the control group. The mass of the heart, liver and muscle was 14.4 % (p <0.001), 20.9 % (p <0.001) and 3.1 %, respectively; in the second group, 1.9 %, 14.7 % (p <0.001) and 1.8 %; in the third group, 29.8 % (p <0.001), 21.5 % (p <0.001) and 5.3 % (p <0.05).

The research work is devoted to the analysis of existing experimental and methodological approaches to the study of immunocorrecting capabilities of local action by preformed physical factors on immunologically significant functionally active zones of the organism in laboratory animals. In an experiment on guinea pigs, it was shown that the local effect of decimeter waves on the thymus causes a reduction in the relative amount of T-lymphocytes in the lymphoid organs and blood, inhibits both the IgM and IgG antibody production to erythrocytes of the ram, and also the production of homocytotrophic antibodies in sensitization with antigen of staphylococcus Gowan-1. Analysis of literature data showed that the effect of immunomodulation of microwaves is reproduced in traditional laboratory animals, laboratory rodents, representatives of new species of laboratory animals. This makes it possible to characterize this phenomenon as an immunobiological one. Accordingly, ongoing scientific work is characterized as research in physiotherapeutic immunology.

In the work morphometric indices of hepatocytes in turkeys were studied when mineral additives were added to the diet. Studies were conducted on turkeys, according to the principle of pairs of analogs, two groups of birds were formed with 20 heads in each group. The first group with the main diet received a zeolite-containing rock of the Atyashevsky field of the Republic of Mordovia and a coniferous energy supplement in an amount of 4% of each drug. The second group was the control. The microstructure of the turkeys liver in different age periods has own features. Microscopy of liver samples in the experimental group revealed that the border between individual hepatocytes is better expressed than in the control group. Reducing the size of hepatocytes with the use of mineral supplements, in contrast to the control group, prevents the development of granular dystrophy.