No 3 (2015)
5-10 96
Abstract
The aim of this work was to study the characteristics of the epizootic and epidemic situation of associative gastrointestinal diseases bacterial etiology of young farm animals, especially calves, in the Rostov region. During the epizootic studies have found that cattle, and rarely pigs are the main reservoir and the source of mixed infections. The study of the spread of gastrointestinal mixed infection showed that in recent years they have recorded in the Rostov region permanently, mainly in administrative areas with high anthropogenic load. At the same time, the disease has stationary character in nature, periodically appearing in the same farms as a massive epizootic outbreaks among young animals first days their life, and characterized by a high percentage of morbidity and mortality. An analysis of the proportion of associative gastrointestinal infections over the past 5 years has shown that they occupy the 4th place among all the bacterial disease of cattle, and their share is 9.3%. At the same time it found a pronounced seasonality of the disease. Most cases of mixed infections in calves lays to the period from November to March with a peak in February
VECTOR DOGS INFECTION, CLINICAL AND MORPHOLOGICAL ASPECTS OF BABESIOSIS IN DOGS IN THE ROSTOV REGION
10-16 56
Abstract
The aim of this study was to determine the presence of babesiosis in dogs in the Rostov region, find the vectors of transmit babesiosis and determine the Babesia species in the region. According to foreign authors, the carrier of infection can lead to autoimmune anemia, to kidney damage and to the development of a fatal kidney failure. Diagnosis by light microscopy such animals is impossible. It was found that the carrier of babesiosis in dogs in the Rostov region at the end of infection transmission consist 12%, while the incidence of carrier B. canis consist 9% of all animals or 75% - of the number of carriers. The mixed invasion of B. canis and B. vogeli was 4% of all animals tested, or 33.3% - of the number of carriers. In 25% of cases to identify the type of Babesia failed.
16-21 77
Abstract
The article presents some aspects of the methodology for the differential diagnosis of swine diseases of bacterial etiology. Isolation of pathogenic S. cholerae suis from pigs of various organs showed that the largest number of pathogenic strains of Salmonella can be isolated from the gallbladder - 30.3%, from the liver in only 14.5% of cases, kidney and spleen - 13.1 % heart - 9.2%. In the study on salmonella it is necessary to make the crops from the gall bladder to extract «pure culture». The microorganisms sensitivity in drug is differented with different organs. Thus, microorganisms isolated from the blood of the heart, characterized by the highest stability. They were sensitive only to four of the 32 drugs, whereas the microorganisms isolated from other organs, are sensitive to 6-15 drug substances. Therefore, research in the allocation of microorganisms should be carried out in different organs and tissues based on localization of the pathogen, and pharmacokinetics of drugs
22-31 105
Abstract
State and public organizations of Russia (Soviet Union) and many European countries have been taken serious and costly projects to preserve of bison and returning animals to the places of their historical habitat. Now active steps are carried out for the reintroduction of species too and to developing a competent bison conservation strategy is required an understanding of the specific reasons hampering the development of populations in conditions conducive to increasing the number. An important role for the survival of the population plays a stability of epizootic situation. The study of the spectrum of bisons pathogens and the epidemiological value is especially important in the context of the reintroduction of grown in the nursery of animals to the natural habitat. Among diseases of viral etiology bison were noted: rabies, bluetongue, viral diarrhea, infectious bovine rhinotracheitis, malignant catarrhal fever, leukemia, neonatal diarrhea, parainfluenza-3, foot and mouth disease. Was given epizootic data, clinical signs, diagnosis and treatment of diseases
31-37 57
Abstract
The paper is devoted to the study of infected dogs by Demodex canis mites in Vladivostok. During the period from December 2013 to May 2015 were examined 54 dogs with skin disease of
74-80 149
Abstract
The article presents the history of the chair of veterinary-sanitary examination of Voronezh State Agrarian University named Emperor Peter 1 from its inception to the present. It was considering the main activities of faculty: educational, scientific, educational. History of the department is closely connected with the history of veterinary education in Russia and the history of the faculty of veterinary medicine and livestock technology. The educational process aims to prepare graduates as the experts capable of carrying out veterinary-sanitary inspection, biological, microbiological, toxicological, chemical and other examinations of safety and quality of raw materials and products of animal and plant origin at all levels of the production. The uniqueness of the training of students at the chair lays in close relation of the educational process, science and real production. Teachers of the chair conduct research on topics: «Animal health and toxic-ecological methodology to ensure the usefulness, quality and safety of raw materials and products of animal origin.» Teaching staff of the chair cherishes the traditions of scientific schools of Voronezh agrarian university, continues communication with teachers which long time worked in the chairand gave a lot of work, effort and effort for the benefit of veterinary science and education.
МОРФОЛОГИЯ И ПАТОЛОГИЧЕСКАЯ АНАТОМИЯ
59-64 68
Abstract
The aim of this work was to study of the ultramicroscopic structure of the blood-brain barrier from broiler chickens of different ages. To study the brain was taken brain of broiler chickens 1, 20 and 38 days old. After studies can draw the following conclusion: after hatching chicken the blood-brain barrier has fully formed structure. Such barrier components as the basement membrane, endothelial cells, glial «clutch» are present. Endothelial cells have a close contact with each other. The layer of the basement membrane is structured. Glial cells surround the vessels tightly. Further barrier structure is maintained. The elements are becoming more structured. Pericytes are viewed. Nevertheless, marked perivascular edema and enlightenment in the cytoplasm of astrocyte ends. In the finishing period of the productivity of broiler chickens the blood-brain barrier has not lost its morphological identity. Endothelial cells become are larger, sometimes swollen. Regarding glial cell, they show signs of degradation in the form of cleaning of nucleoplasm and destruction of the internal structure. Furthermore, there is perivascular edema, which indicates the pathological processes in the structure of the blood-brain barrier.
МИКРОБИОЛОГИЯ
43-49 53
Abstract
The aim of research was to improve the methodology of identification of Pasteurella multocida in the blood of infected and dead birds on the basis of microscopy. In numerous experiments have used 11 strains of the most common among birds. There were serovar A:1 P. multocida: X-73 (from the collection of Heddlestouna, USA), control strains: 55, 115, 712, 915, 1931 (VGNKI veterinary products) and 5 virulent natural ones. Morphogenesis of bacteria in the organism and poultry carcass has studied depending on the route of contamination, the contaminating material, the temperature and the liquid phase environment. The research results served as the basis of significantly changing of traditional idea of the morphology of P. multocida cells. It is shown at the level of light and electron microscopies (including the formation of the capsule and bipolarity) P. multocida shaping in the dynamics in the body and the corpse. For the first time found conditions for the formation of the capsule, the explanation of the phenomenon of bipolarity and of explaining the functional morphology and thermolability of P. multocida. It is shown that temperature is a factor of influence on P. multocida capsule forming, changes the morphology of the bacteria and leads to its penetration into the body of a bird. Compared with the traditional options presented methodology is distinguished by objective research, the reliability of the result, minimizing material costs and labor time. The aim of research was to improve the methodology of identification of Pasteurella multocida in the blood of infected and dead birds on the basis of microscopy. In numerous experiments have used 11 strains of the most common among birds. There were serovar A:1 P. multocida: X-73 (from the collection of Heddlestouna, USA), control strains: 55, 115, 712, 915, 1931 (VGNKI veterinary products) and 5 virulent natural ones. Morphogenesis of bacteria in the organism and poultry carcass has studied depending on the route of contamination, the contaminating material, the temperature and the liquid phase environment. The research results served as the basis of significantly changing of traditional idea of the morphology of P. multocida cells. It is shown at the level of light and electron microscopies (including the formation of the capsule and bipolarity) P. multocida shaping in the dynamics in the body and the corpse. For the first time found conditions for the formation of the capsule, the explanation of the phenomenon of bipolarity and of explaining the functional morphology and thermolability of P. multocida. It is shown that temperature is a factor of influence on P. multocida capsule forming, changes the morphology of the bacteria and leads to its penetration into the body of a bird. Compared with the traditional options presented methodology is distinguished by objective research, the reliability of the result, minimizing material costs and labor time.
ИМУННОЛОГИЯ
37-43 89
Abstract
The article presents data on the assessment of the immune status of reindeer. The aim of the study was to investigate the characteristics of animal blood immunological parameters depending on age and season of the year (1 group of animals is 2-3 years old, 2 - 4-6 years, 3 - 7-9 years, 4 - 10 years and older). The material of the study are samples of whole blood and blood serum. Hematologic parameters were determined. Also was got leucocytes differential count, total number of immunoglobulins, total protein and functional activity of phagocytes. Analysis of the results of the study showed that summer characterizes by low levels of functional activity of neutrophils (18,12-25,7%) and serum total protein content (60,63-87,31 g/l), decreasing the level of hemoglobin, red blood cell count (4,37-9,59.1012/l) and hematocrit (20,1-36,4%) for the animals regardless of age. Normalization of these parameters is observed in autumn. In autumn pronounced age differences of the immune status of reindeer: low level of functional activity of neutrophils (19.39%), total protein (64.81 g/L) and total immunoglobulin (7.04 mg/ml) in the serum of 1 groups deer, which increases the likelihood of seasonal animal diseases in this age group
ТЕРАПИЯ
49-54 104
Abstract
Comprehensive therapy with ASD fraction 2 and Hydro Electric Vital at transport stress in pigeons in the Salsk district of Rostov region was controlled of clinical examination of poultry, laboratory tests of blood (hematology and biochemistry). Pigeons from 1 and 2 experimental groups was subjected by transportign over a distance of 400km and 180 km with a further flight over the same distance, revealed the following changes: weight loss, rise in body temperature, increased respiratory movements, tachycardia, diarrhea, increased levels of hemoglobin, hematocrit, the CPU, the number of erythrocytes, leukocytes, and platelets, glucose, blood pH, lowering the ESR, total protein, albumin, uric acid. To birds of groups 3 and 4 was given ASD fraction 2 and Hydro Electric Vital in 4 days before and 2 days after transport. The drugs revealed positive a shift in clinical status, hematological and biochemical parameters of the birds.
РАДИОБИОЛОГИЯ
55-59 82
Abstract
The aim of this work was to study the effectiveness of the impact of a natural absorber on the rate of excretion from the mice body 137Cs retaining their physiological state. We studied two types of absorbent preparations of natural origin: radium and zeolite. The effectiveness of the impact of these substances on the rate of excretion from the body 137 Cs mice with a view to preserving normal physiological state of animals was evaluated. It was found that the use of sorbents for the removal of radionuclides from the body of animals have no negative effect on the body. The effectiveness of sorbents was estimated by two parameters. Was estimated percentage of cesium removal from the body of animals, and also the estimated rate of excretion of radioactive cesium from the body in the application of study medication. To achieve this goal was used gamma-spectrometric method for the determination of radiocaesium in the organs and tissues of animals. The percentage of cesium removal from the body when using radionita was 90%. The percentage of cesium removal from the body, using a zeolite was 75.9%. The biological half-life of radioactive cesium from the body when using radionita is 1 day. The biological half-life of radioactive cesium from the body when using a zeolite is 4.5 days. The research results can be used for the in the breeding of livestock in areas with radioactive contamination
САНИТАРИЯ И ЗООГИГИЕНА
65-69 62
Abstract
The aim of this study was to investigate the efficiency of photocatalytic method disinfection of indoor air for rearing quails. It was found that after application of photocatalysis the total microbial contamination of air on the 15th day of cultivation was decreased by 1.5 times as compared with startof the seating. The total content of microorganisms on the 30th day of cultivation using photocatalysis was 1.7 times lower than in the period with start of the seating, and 1.8 times as compared with the period in a day after seating. Contamination of air by enterobacteria after using photocatalysis on the 15th day of the test was decreased by 1.5 times. Contents of enterobacteria in inside air on the 30th day of cultivation whith the processing method of the photocatalyst was 2.0 times lower than in the period after start of the seating, and 4.4 times as compared with the period in a day after seating. Fungi contamination of air after using photocatalysis on the 15th day of the test decreased by 1.9 times in comparison with the index after start of the seating, and 1.1 times as compared with a day after the seating. The content of fungi in indoor air on the 30th day of cultivation after method of the photocatalyst decreased 2-fold compared to the period after starting seating, and 1.2 times as compared with the period of a day after seating. Safety and weight gain the birds in the experimental group was 92%, and 169.5g. In the control group these parametres were 87.2% and 140,0g.
69-74 57
Abstract
The article is devoted to the comparative efficacy of disinfectants in poultry. The use of modern effective means of disinfection plays an important role in ensuring of the bacteriological safety on poultry farms. In the context of a wide variety of disinfectants, there is a problem of choosing the most effective drugs, taking into account economic efficiency, as well as the specific features of the poultry sector. On the poultry farm Markinskaya conducted experiment to determine the relative effectiveness of disinfectants of the three different groups: 1. products based on chlorine, phenol, formaldehyde (Dezkonten, bleach), 2. products based on peracetic acid (NAA Dezoksid), 3. combination of drugs on the basis of a Quaternary ammonium compounds (Virudez MAX). 100% efficacy against coliform bacteria, group of staph bacteria, mesophilic aerobic and facultative anaerobic microorganisms showed preparations: Virudez MAX, Dezoksid NUK, Dezkonten. Experience has shown that the most effective disinfectant proved to be a combined preparation Virudez MAX. The test results are consistent with those of trials of the other drug in this group on the livestock farms of the Moscow Region and the Republic of Dagestan.
ISSN 2949-4826 (Online)